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A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot and mouth disease in District Faisalabad, Pakistan during the Year 2016

Author

Listed:
  • Waqas Ali

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Mudasser Habib

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Sanaullah Sajid

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Rai Shafqat Ali Khan

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • M. Usman Mazhar

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Irfan Ullah Khan

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Uneeb Saliha

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Muhammad Farooq

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Irfan Ullah Khan

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Uneeb Saliha

    (Institute of Microbiology, Faculty of veterinary science, University of Agriculture, Faisalabad, Pakistan)

  • Muhammad Farooq

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • M. Salah Ud Din Shah

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

  • Hafiz Muhammad Muzammil

    (Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan)

Abstract

Foot and mouth disease is an economically devastating disease of livestock that mainly effect cloven-hoofed animals i.e. sheep, goat, cattle, pig, buffalo, deer etc. The aim of this study was to determine the serotypes circulating in the region during 2016. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the confirmation of FMDV genome. Out of total 72 samples, 65 were found positive which were then serotyped into type O (n=30), Asia1 (n=19) and A (n=5). Some samples (n=5) were found positive for more than one serotype that were subjected to reverse transcriptase loop-mediated isothermal amplification assay (RT-LAMP) for serotype determination.

Suggested Citation

  • Waqas Ali & Mudasser Habib & Sanaullah Sajid & Rai Shafqat Ali Khan & M. Usman Mazhar & Irfan Ullah Khan & Uneeb Saliha & Muhammad Farooq & Irfan Ullah Khan & Uneeb Saliha & Muhammad Farooq & M. Salah, 2017. "A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot and mouth disease in District Faisalabad, Pakistan during the Year 2016," Matrix Science Medica (MSM), Zibeline International Publishing, vol. 1(1), pages 27-29, January.
    • Handle: RePEc:zib:zbnmsm:v:1:y:2017:i:1:p:27-29
    DOI: 10.26480/msm.01.2017.27.29
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    Cited by:

    1. Waqas Ali & Mudasser Habib & Sanaullah Sajid & Rai Shafqat Ali Khan & Muhammad Ashir Zia & Irfan Ullah Khan & Asad Javed & Hafiz Muhammad Muzammil & Muhammad Usman Mazhar & Muhammad Farooq & Muhammad , 2017. "Comparison And Evaluation Of OneStep Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) And Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay(RTLAMP) For Rapid Detection Of Foot," Matrix Science Medica (MSM), Zibeline International Publishing, vol. 1(2), pages 16-19, January.

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