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High-Quality Dna From Peat Soil For Metagenomic Studies: A Minireview On Dna Extraction Methods

Author

Listed:
  • Muhamad Aidilfitri Mohamad Roslan

    (Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Malaysia)

  • Mohd Azrul Naim Mohamad

    (Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Malaysia)

  • Suhaila Mohd Omar

    (Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Malaysia)

Abstract

The understanding of microorganism’s biodiversity in the peatland soil through non-cultivation based approach provide important inputs towards prediction of the ecosystem response towards the changing environment. The challenge that hindered the success to obtain high quality DNA from peat soil lies in the physicochemical characteristics such as low pH and high humic acid content. There are two general approaches that have been extensively applied for soil microbial DNA extraction, which are direct DNA extraction protocol and indirect DNA extraction protocol. The only step differentiate between these two protocols is the later includes additional cell separation method from soil micro-aggregates preceding DNA extraction process. Therefore, several improved and modified methods in conventional DNA extraction and purification methods are reviewed in this paper to cater all the highlighted issues as to obtain high-quality DNA for peatland metagenomics soil studies.

Suggested Citation

  • Muhamad Aidilfitri Mohamad Roslan & Mohd Azrul Naim Mohamad & Suhaila Mohd Omar, 2017. "High-Quality Dna From Peat Soil For Metagenomic Studies: A Minireview On Dna Extraction Methods," Science Heritage Journal (GWS), Zibeline International Publishing, vol. 1(2), pages 1-6, January.
    • Handle: RePEc:zib:zbngws:v:1:y:2017:i:2:p:1-6
    DOI: 10.26480/gws.02.2017.01.06
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