IDEAS home Printed from https://ideas.repec.org/a/wsi/srlxxx/v23y2016i02ns0218625x15501036.html
   My bibliography  Save this article

A Graphene/Enzyme-Based Electrochemical Sensor For Sensitive Detection Of Organophosphorus Pesticides

Author

Listed:
  • NONG ZHOU

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • CHENGYONG LI

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • RIJIAN MO

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • PENG ZHANG

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • LEI HE

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • FANGHONG NIE

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • WEIMING SU

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • SHUCHENG LIU

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • JING GAO

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • HAIYAN SHAO

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

  • ZHONG-JI QIAN

    (#x2020;Department of Chemistry, Pukyong National University, Busan 608737, Korea)

  • HONGWU JI

    (College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key, Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, P. R. China)

Abstract

A sensitive and fast sensor for quantitative detection of organophosphorus pesticides (OPs) is obtained using acetylcholinesterase (AChE) biosensor based on graphene oxide (GO)–chitosan (CS) composite film. This new biosensor is prepared via depositing GO–CS composite film on glassy carbon electrode (GCE) and then assembling AChE on the composite film. The GO–CS composite film shows an excellent biocompatibility with AChE and enhances immobilization efficiency of AChE. GO homogeneously disperses in the GO–CS composite films and exhibits excellent electrocatalytic activity to thiocholine oxidation, which is from acetylthiocholine catalyzed by AChE. The results show that the inhibition of carbaryl/trichlorfon on AChE activity is proportional to the concentration of carbaryl/trichlorfon. The detection of linear range for carbaryl is from 10nM to 100nM and the correlation coefficients of 0.993. The detection limit for carbaryl is calculated to be about 2.5nM. In addition, the detection of linear range for trichlorfon is from 10nM to 60nM and the correlation coefficients of 0.994. The detection limit for trichlorfon is calculated to be about 1.2nM. This biosensor provides a new promising tool for trace organophosphorus pesticide detection.

Suggested Citation

  • Nong Zhou & Chengyong Li & Rijian Mo & Peng Zhang & Lei He & Fanghong Nie & Weiming Su & Shucheng Liu & Jing Gao & Haiyan Shao & Zhong-Ji Qian & Hongwu Ji, 2016. "A Graphene/Enzyme-Based Electrochemical Sensor For Sensitive Detection Of Organophosphorus Pesticides," Surface Review and Letters (SRL), World Scientific Publishing Co. Pte. Ltd., vol. 23(02), pages 1-8, April.
    • Handle: RePEc:wsi:srlxxx:v:23:y:2016:i:02:n:s0218625x15501036
    DOI: 10.1142/S0218625X15501036
    as

    Download full text from publisher

    File URL: http://www.worldscientific.com/doi/abs/10.1142/S0218625X15501036
    Download Restriction: Access to full text is restricted to subscribers
    File URL: https://libkey.io/10.1142/S0218625X15501036?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:wsi:srlxxx:v:23:y:2016:i:02:n:s0218625x15501036. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Tai Tone Lim (email available below). General contact details of provider: http://www.worldscinet.com/srl/srl.shtml .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.