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Visualizationex Situof Single Dna Molecules Incubation: A First Step For Quantitative Analysis On Multi-Site Degradation And Enzymatic Kinetics

Author

Listed:
  • XINYAN WANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
    Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China)

  • HAIJUN YANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
    Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China)

  • HUABIN WANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China)

  • PENG WANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China)

  • HAI LI

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China)

Abstract

Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNAin vitrotests using DNase I incubation based on high-resolution AFM imagingex situwith fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation and enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension and strong immobilization on a surface and discover the mechanochemistry.

Suggested Citation

  • Xinyan Wang & Haijun Yang & Huabin Wang & Peng Wang & Hai Li, 2009. "Visualizationex Situof Single Dna Molecules Incubation: A First Step For Quantitative Analysis On Multi-Site Degradation And Enzymatic Kinetics," Surface Review and Letters (SRL), World Scientific Publishing Co. Pte. Ltd., vol. 16(01), pages 79-85.
    • Handle: RePEc:wsi:srlxxx:v:16:y:2009:i:01:n:s0218625x09012329
    DOI: 10.1142/S0218625X09012329
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