Alterations in aerobic and anaerobic dehydrogenases and protein levels of three tropical earthworm species with respect to different seasons

The specific activities of cMDH, mMDH and LDH of earthworms (M. posthuma, P. sansibaricus, L. mauritii) started increasing with the onset of favorable seasonal conditions from July to August. A lower temperature and moisture favor the increase in specific activities of enzymes during post-rainy (September–October) and winter (November–January) seasons. The maximum specific activities of enzymes from December to January indicate greater aerobic and anaerobic energy production to cope up with the cold condition. However, the enzyme activities decreased in summer (February–April) with the increase in the ambient temperature. The specific activities were minimum in summer (May–June) as earthworms would be entering quiescent phase to avoid extreme heat and thus showed least energy requirement in this period. Similar seasonal variations were found in the cytoplasmic and mitochondrial proteins. The maximum and minimum seasonal effects were on the epigeic (P. sansibaricus) and endo-anecic (M. posthuma) earthworms, respectively. The differences in the profile of dehydrogenases and proteins may be assigned to the differences in the ecological categories of earthworms.