IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0252426.html
   My bibliography  Save this article

Differences in levels of phosphatidylinositols in healthy and stable Coronary Artery Disease subjects revealed by HILIC-MRM method with SERRF normalization

Author

Listed:
  • Yue Huang
  • Ruipeng Mu
  • David Wen
  • Joseph S Grimsby
  • Meina Liang
  • Anton I Rosenbaum

Abstract

Quantification of endogenous biomarkers in clinical studies requires careful evaluation of a number of assay performance parameters. Comparisons of absolute values from several clinical studies can enable retrospective analyses further elucidating the biology of a given biomarker across various study populations. We characterized the performance of a highly multiplex bioanalytical method for quantification of phosphatidylinositols (PI). Hydrophilic interaction chromatography (HILIC) and multiple reaction monitoring (MRM) were employed for targeted multiplex quantification. Odd-chain PI species that are not normally present in human plasma were utilized as surrogate analytes (SA) to assess various assay performance parameters and establish a definitive dynamic linear range for PI lipids. To correct for batch effects, Systematic Error Removal using Random Forest (SERRF) normalization algorithm was employed and used to bridge raw values between two clinical studies, enabling quantitative comparison of their absolute values. A high throughput method was developed, qualified, transferred to an automation platform and applied to sample testing in two clinical trials in healthy volunteers (NCT03001297) and stable Coronary Artery Disease (CAD, NCT03351738) subjects. The method demonstrated acceptable precision and accuracy (±30%) over linear range of 1–1000 nM for SA and 8-fold dilutional linearity for endogenous PI. We determined that mean-adjusted average QC performed best for normalization using SERRF. The comparison of two studies revealed that healthy subject levels of PI are consistently higher across PI species compared to CAD subjects identifying a potential lipid biomarker to be explored in future studies.

Suggested Citation

  • Yue Huang & Ruipeng Mu & David Wen & Joseph S Grimsby & Meina Liang & Anton I Rosenbaum, 2021. "Differences in levels of phosphatidylinositols in healthy and stable Coronary Artery Disease subjects revealed by HILIC-MRM method with SERRF normalization," PLOS ONE, Public Library of Science, vol. 16(6), pages 1-14, June.
    • Handle: RePEc:plo:pone00:0252426
    DOI: 10.1371/journal.pone.0252426
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252426
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0252426&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0252426?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Gilbert Di Paolo & Pietro De Camilli, 2006. "Phosphoinositides in cell regulation and membrane dynamics," Nature, Nature, vol. 443(7112), pages 651-657, October.
    2. Xinchun Zhou & Jinghe Mao & Junmei Ai & Youping Deng & Mary R Roth & Charles Pound & Jeffrey Henegar & Ruth Welti & Steven A Bigler, 2012. "Identification of Plasma Lipid Biomarkers for Prostate Cancer by Lipidomics and Bioinformatics," PLOS ONE, Public Library of Science, vol. 7(11), pages 1-11, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Antonio L Egea-Jiménez & Ángel Pérez-Lara & Senena Corbalán-García & Juan C Gómez-Fernández, 2013. "Phosphatidylinositol 4,5-Bisphosphate Decreases the Concentration of Ca2+, Phosphatidylserine and Diacylglycerol Required for Protein Kinase C α to Reach Maximum Activity," PLOS ONE, Public Library of Science, vol. 8(7), pages 1-8, July.
    2. Maria Thürmer & André Gollowitzer & Helmut Pein & Konstantin Neukirch & Elif Gelmez & Lorenz Waltl & Natalie Wielsch & René Winkler & Konstantin Löser & Julia Grander & Madlen Hotze & Sönke Harder & A, 2022. "PI(18:1/18:1) is a SCD1-derived lipokine that limits stress signaling," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    3. Nilmani Singh & Adriana Reyes-Ordoñez & Michael A. Compagnone & Jesus F. Moreno & Benjamin J. Leslie & Taekjip Ha & Jie Chen, 2021. "Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    4. Darshini Jeyasimman & Bilge Ercan & Dennis Dharmawan & Tomoki Naito & Jingbo Sun & Yasunori Saheki, 2021. "PDZD-8 and TEX-2 regulate endosomal PI(4,5)P2 homeostasis via lipid transport to promote embryogenesis in C. elegans," Nature Communications, Nature, vol. 12(1), pages 1-21, December.
    5. Salome Funes & Jonathan Jung & Del Hayden Gadd & Michelle Mosqueda & Jianjun Zhong & Shankaracharya & Matthew Unger & Karly Stallworth & Debra Cameron & Melissa S. Rotunno & Pepper Dawes & Megan Fowle, 2024. "Expression of ALS-PFN1 impairs vesicular degradation in iPSC-derived microglia," Nature Communications, Nature, vol. 15(1), pages 1-25, December.
    6. Seiichi Koike & Reinhard Jahn, 2024. "Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    7. Yu-Te Yeh & Chandan Sona & Xin Yan & Yunxiao Li & Adrija Pathak & Mark I. McDermott & Zhigang Xie & Liangwen Liu & Anoop Arunagiri & Yuting Wang & Amaury Cazenave-Gassiot & Adhideb Ghosh & Ferdinand v, 2023. "Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    8. Di-Ao Liu & Kai Tao & Bin Wu & Ziyan Yu & Malwina Szczepaniak & Matthew Rames & Changsong Yang & Tatyana Svitkina & Yueyao Zhu & Fengyuan Xu & Xiaolin Nan & Wei Guo, 2023. "A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0252426. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.