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Fiber stiffness, pore size and adhesion control migratory phenotype of MDA-MB-231 cells in collagen gels

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  • Florian Geiger
  • Daniel Rüdiger
  • Stefan Zahler
  • Hanna Engelke

Abstract

Cancer cell migration is influenced by cellular phenotype and behavior as well as by the mechanical and chemical properties of the environment. Furthermore, many cancer cells show plasticity of their phenotype and adapt it to the properties of the environment. Here, we study the influence of fiber stiffness, confinement, and adhesion properties on cancer cell migration in porous collagen gels. Collagen gels with soft fibers abrogate migration and promote a round, non-invasive phenotype. Stiffer collagen fibers are inherently more adhesive and lead to the existence of an adhesive phenotype and in general confined migration due to adhesion. Addition of TGF-β lowers adhesion, eliminates the adhesive phenotype and increases the amount of highly motile amoeboid phenotypes. Highest migration speeds and longest displacements are achieved in stiff collagen fibers in pores of about cell size by amoeboid phenotypes. This elucidates the influence of the mechanical properties of collagen gels on phenotype and subsequently migration and shows that stiff fibers, cell sized pores, and low adhesion, are optimal conditions for an amoeboid phenotype and efficient migration.

Suggested Citation

  • Florian Geiger & Daniel Rüdiger & Stefan Zahler & Hanna Engelke, 2019. "Fiber stiffness, pore size and adhesion control migratory phenotype of MDA-MB-231 cells in collagen gels," PLOS ONE, Public Library of Science, vol. 14(11), pages 1-13, November.
    • Handle: RePEc:plo:pone00:0225215
    DOI: 10.1371/journal.pone.0225215
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    1. Tim Lämmermann & Bernhard L. Bader & Susan J. Monkley & Tim Worbs & Roland Wedlich-Söldner & Karin Hirsch & Markus Keller & Reinhold Förster & David R. Critchley & Reinhard Fässler & Michael Sixt, 2008. "Rapid leukocyte migration by integrin-independent flowing and squeezing," Nature, Nature, vol. 453(7191), pages 51-55, May.
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