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Evaluation of alternately combining HPV viral load and 16/18 genotyping in secondary screening algorithms

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  • Hongxue Luo
  • Hui Du
  • Jerome L Belinson
  • Ruifang Wu

Abstract

Background: Correlation with HPV viral load and worsening cervical lesions had been reported, but its potential for triage after primary HPV screening has not been adequately explored, especially when combined with HPV-16/18 genotyping. Objective: To evaluate combinations of human papillomavirus (HPV) viral load and genotyping for HPV-16/18 as secondary screening strategies. Methods: The Shenzhen Cervical Cancer Screening Trial Ⅱ (SHENCCAST Ⅱ) database was re-analyzed to explore new screening algorithms using the results of Hybrid Capture 2 (HC2), Mass Array Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass spectrometry System (MALDI-TOF-MS) and the ThinPrep cytologic test (TCT) obtained by endocervical sampling. Results: Compared with the recommended screening strategy of genotyping HPV-16/18 plus reflex to cytology, using viral load (10 RLU/CO as threshold) plus reflex to cytology resulted in less cytology but had a significantly higher sensitivity for cervical intraepithelial neoplasia 2+ (CIN2+)/CIN3+ without considerable changes in specificity and referral rates. Both of the strategy of using viral load ≥10 RLU/CO as cut-point for immediate colposcopy followed by triage genotyping HPV-16/18 for the other positive (≥1

Suggested Citation

  • Hongxue Luo & Hui Du & Jerome L Belinson & Ruifang Wu, 2019. "Evaluation of alternately combining HPV viral load and 16/18 genotyping in secondary screening algorithms," PLOS ONE, Public Library of Science, vol. 14(7), pages 1-9, July.
    • Handle: RePEc:plo:pone00:0220200
    DOI: 10.1371/journal.pone.0220200
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