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APNG as a prognostic marker in patients with glioblastoma

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Listed:
  • Sigurd Fosmark
  • Sofie Hellwege
  • Rikke H Dahlrot
  • Kristian L Jensen
  • Helene Derand
  • Jesper Lohse
  • Mia D Sørensen
  • Steinbjørn Hansen
  • Bjarne W Kristensen

Abstract

Aim: Expression of the base excision repair enzyme alkylpurine-DNA-N-glycosylase (APNG) has been correlated to temozolomide resistance. Our aim was to evaluate the prognostic value of APNG in a population-based cohort with 242 gliomas including 185 glioblastomas (GBMs). Cellular heterogeneity of GBMs was taken into account by excluding APNG expression in non-tumor cells from the analysis. Methods: APNG expression was evaluated using automated image analysis and a novel quantitative immunohistochemical (IHC) assay (qIHC), where APNG protein expression was evaluated through countable dots. Non-tumor cells were excluded using an IHC/qIHC double-staining. For verification, APNG was measured by a quantitative double-immunofluorescence (IF) assay. As validation APNG mRNA expression was evaluated using independent TCGA data. Results: Using qIHC, high levels of APNG were associated with better overall survival (OS) in univariate (HR = 0.50; P

Suggested Citation

  • Sigurd Fosmark & Sofie Hellwege & Rikke H Dahlrot & Kristian L Jensen & Helene Derand & Jesper Lohse & Mia D Sørensen & Steinbjørn Hansen & Bjarne W Kristensen, 2017. "APNG as a prognostic marker in patients with glioblastoma," PLOS ONE, Public Library of Science, vol. 12(6), pages 1-17, June.
    • Handle: RePEc:plo:pone00:0178693
    DOI: 10.1371/journal.pone.0178693
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