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Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB)

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  • Gayathri N Sekhar
  • Ana R Georgian
  • Lisa Sanderson
  • Gema Vizcay-Barrena
  • Rachel C Brown
  • Paula Muresan
  • Roland A Fleck
  • Sarah A Thomas

Abstract

Pentamidine is an effective trypanocidal drug used against stage 1 Human African Trypanosomiasis (HAT). At the blood-brain barrier (BBB), it accumulates inside the endothelial cells but has limited entry into the brain. This study examined transporters involved in pentamidine transport at the human and mouse BBB using hCMEC/D3 and bEnd.3 cell lines, respectively. Results revealed that both cell lines expressed the organic cation transporters (OCT1, OCT2 and OCT3), however, P-gp was only expressed in hCMEC/D3 cells. Polarised expression of OCT1 was also observed. Functional assays found that ATP depletion significantly increased [3H]pentamidine accumulation in hCMEC/D3 cells (***p

Suggested Citation

  • Gayathri N Sekhar & Ana R Georgian & Lisa Sanderson & Gema Vizcay-Barrena & Rachel C Brown & Paula Muresan & Roland A Fleck & Sarah A Thomas, 2017. "Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB)," PLOS ONE, Public Library of Science, vol. 12(3), pages 1-26, March.
    • Handle: RePEc:plo:pone00:0173474
    DOI: 10.1371/journal.pone.0173474
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