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Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame

Author

Listed:
  • Takashi Ohtsuki
  • Ryoichiro Nakamura
  • Satoru Kubo
  • Akira Otabe
  • Yoko Oobayashi
  • Shoko Suzuki
  • Mika Yoshida
  • Mitsuya Yoshida
  • Chiye Tatebe
  • Kyoko Sato
  • Hiroshi Akiyama

Abstract

α-L-Aspartyl-D-phenylalanine methyl ester (L, D-APM) and α-D-aspartyl-L-phenylalanine methyl ester (D, L-APM) are diastereomers of aspartame (N-L-α-Aspartyl-L-phenylalanine-1-methyl ester, L, L-APM). The Joint FAO/WHO Expert Committee on Food Additives has set 0.04 wt% as the maximum permitted level of the sum of L, D-APM and D, L-APM in commercially available L, L-APM. In this study, we developed and validated a simple high-performance liquid chromatography (HPLC) method using an ODS column to determine L, D-APM and D, L-APM in L, L-APM. The limits of detection and quantification, respectively, of L, D-APM and D, L-APM were found to be 0.0012 wt% and 0.004 wt%. This method gave excellent accuracy, repeatability, and reproducibility in a recovery test performed on five different days. Moreover, the method was successfully applied to the determination of these diastereomers in commercial L, L-APM samples. Thus, the developed method is a simple, useful, and practical tool for determining L, D-APM and D, L-APM levels in L, L-APM.

Suggested Citation

  • Takashi Ohtsuki & Ryoichiro Nakamura & Satoru Kubo & Akira Otabe & Yoko Oobayashi & Shoko Suzuki & Mika Yoshida & Mitsuya Yoshida & Chiye Tatebe & Kyoko Sato & Hiroshi Akiyama, 2016. "Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame," PLOS ONE, Public Library of Science, vol. 11(3), pages 1-8, March.
    • Handle: RePEc:plo:pone00:0152174
    DOI: 10.1371/journal.pone.0152174
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