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Quality Evaluation of Potentilla fruticosa L. by High Performance Liquid Chromatography Fingerprinting Associated with Chemometric Methods

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  • Wei Liu
  • Dongmei Wang
  • Jianjun Liu
  • Dengwu Li
  • Dongxue Yin

Abstract

The present study was performed to assess the quality of Potentilla fruticosa L. sampled from distinct regions of China using high performance liquid chromatography (HPLC) fingerprinting coupled with a suite of chemometric methods. For this quantitative analysis, the main active phytochemical compositions and the antioxidant activity in P. fruticosa were also investigated. Considering the high percentages and antioxidant activities of phytochemicals, P. fruticosa samples from Kangding, Sichuan were selected as the most valuable raw materials. Similarity analysis (SA) of HPLC fingerprints, hierarchical cluster analysis (HCA), principle component analysis (PCA), and discriminant analysis (DA) were further employed to provide accurate classification and quality estimates of P. fruticosa. Two principal components (PCs) were collected by PCA. PC1 separated samples from Kangding, Sichuan, capturing 57.64% of the variance, whereas PC2 contributed to further separation, capturing 18.97% of the variance. Two kinds of discriminant functions with a 100% discrimination ratio were constructed. The results strongly supported the conclusion that the eight samples from different regions were clustered into three major groups, corresponding with their morphological classification, for which HPLC analysis confirmed the considerable variation in phytochemical compositions and that P. fruticosa samples from Kangding, Sichuan were of high quality. The results of SA, HCA, PCA, and DA were in agreement and performed well for the quality assessment of P. fruticosa. Consequently, HPLC fingerprinting coupled with chemometric techniques provides a highly flexible and reliable method for the quality evaluation of traditional Chinese medicines.

Suggested Citation

  • Wei Liu & Dongmei Wang & Jianjun Liu & Dengwu Li & Dongxue Yin, 2016. "Quality Evaluation of Potentilla fruticosa L. by High Performance Liquid Chromatography Fingerprinting Associated with Chemometric Methods," PLOS ONE, Public Library of Science, vol. 11(2), pages 1-20, February.
    • Handle: RePEc:plo:pone00:0149197
    DOI: 10.1371/journal.pone.0149197
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    References listed on IDEAS

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    1. Dongmei Wang & Fengyuan He & Zhenjiang Lv & Dengwu Li, 2014. "Phytochemical Composition, Antioxidant Activity and HPLC Fingerprinting Profiles of Three Pyrola Species from Different Regions," PLOS ONE, Public Library of Science, vol. 9(5), pages 1-11, May.
    2. Liu, Yanhuai & Sun, Yanling, 2004. "China traditional Chinese Medicine (TCM) Patent Database," World Patent Information, Elsevier, vol. 26(1), pages 91-96, March.
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