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Reliability of Serum Metabolites over a Two-Year Period: A Targeted Metabolomic Approach in Fasting and Non-Fasting Samples from EPIC

Author

Listed:
  • Marion Carayol
  • Idlir Licaj
  • David Achaintre
  • Carlotta Sacerdote
  • Paolo Vineis
  • Timothy J Key
  • N Charlotte Onland Moret
  • Augustin Scalbert
  • Sabina Rinaldi
  • Pietro Ferrari

Abstract

Objective: Although metabolic profiles have been associated with chronic disease risk, lack of temporal stability of metabolite levels could limit their use in epidemiological investigations. The present study aims to evaluate the reliability over a two-year period of 158 metabolites and compare reliability over time in fasting and non-fasting serum samples. Methods: Metabolites were measured with the AbsolueIDQp180 kit (Biocrates, Innsbruck, Austria) by mass spectrometry and included acylcarnitines, amino acids, biogenic amines, hexoses, phosphatidylcholines and sphingomyelins. Measurements were performed on repeat serum samples collected two years apart in 27 fasting men from Turin, Italy, and 39 non-fasting women from Utrecht, The Netherlands, all participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Reproducibility was assessed by estimating intraclass correlation coefficients (ICCs) in multivariable mixed models. Results: In fasting samples, a median ICC of 0.70 was observed. ICC values were 0.50 in fasting and non-fasting samples, respectively. ICCs were higher in fasting samples that are preferable to non-fasting.

Suggested Citation

  • Marion Carayol & Idlir Licaj & David Achaintre & Carlotta Sacerdote & Paolo Vineis & Timothy J Key & N Charlotte Onland Moret & Augustin Scalbert & Sabina Rinaldi & Pietro Ferrari, 2015. "Reliability of Serum Metabolites over a Two-Year Period: A Targeted Metabolomic Approach in Fasting and Non-Fasting Samples from EPIC," PLOS ONE, Public Library of Science, vol. 10(8), pages 1-10, August.
    • Handle: RePEc:plo:pone00:0135437
    DOI: 10.1371/journal.pone.0135437
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