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Quantitative Fitness Analysis Identifies exo1∆ and Other Suppressors or Enhancers of Telomere Defects in Schizosaccharomyces pombe

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  • Siddharth Narayanan
  • Marion Dubarry
  • Conor Lawless
  • A Peter Banks
  • Darren J Wilkinson
  • Simon K Whitehall
  • David Lydall

Abstract

Synthetic genetic array (SGA) has been successfully used to identify genetic interactions in S. cerevisiae and S. pombe. In S. pombe, SGA methods use either cycloheximide (C) or heat shock (HS) to select double mutants before measuring colony size as a surrogate for fitness. Quantitative Fitness Analysis (QFA) is a different method for determining fitness of microbial strains. In QFA, liquid cultures are spotted onto solid agar and growth curves determined for each spot by photography and model fitting. Here, we compared the two S. pombe SGA methods and found that the HS method was more reproducible for us. We also developed a QFA procedure for S. pombe. We used QFA to identify genetic interactions affecting two temperature sensitive, telomere associated query mutations (taz1Δ and pot1-1). We identify exo1∆ and other gene deletions as suppressors or enhancers of S. pombe telomere defects. Our study identifies known and novel gene deletions affecting the fitness of strains with telomere defects. The interactions we identify may be relevant in human cells.

Suggested Citation

  • Siddharth Narayanan & Marion Dubarry & Conor Lawless & A Peter Banks & Darren J Wilkinson & Simon K Whitehall & David Lydall, 2015. "Quantitative Fitness Analysis Identifies exo1∆ and Other Suppressors or Enhancers of Telomere Defects in Schizosaccharomyces pombe," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-15, July.
    • Handle: RePEc:plo:pone00:0132240
    DOI: 10.1371/journal.pone.0132240
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    References listed on IDEAS

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    1. Jean Charles Nault & Maxime Mallet & Camilla Pilati & Julien Calderaro & Paulette Bioulac-Sage & Christophe Laurent & Alexis Laurent & Daniel Cherqui & Charles Balabaud & Jessica Zucman-Rossi, 2013. "High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions," Nature Communications, Nature, vol. 4(1), pages 1-7, October.
    2. Kyle M. Miller & Ofer Rog & Julia Promisel Cooper, 2006. "Semi-conservative DNA replication through telomeres requires Taz1," Nature, Nature, vol. 440(7085), pages 824-828, April.
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