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Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass-Spectrometry (MALDI-TOF MS) Based Typing of Extended-Spectrum β-Lactamase Producing E. coli – A Novel Tool for Real-Time Outbreak Investigation

Author

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  • Adrian Egli
  • Sarah Tschudin-Sutter
  • Michael Oberle
  • Daniel Goldenberger
  • Reno Frei
  • Andreas F Widmer

Abstract

Epidemiologically linked clusters are confirmed by typing strains with molecular typing such as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-lactamase producing E. coli of a PFGE-related cluster with Matrix-assisted laser desorption/ionization-time of flight mass-spectrometry based typing that confirmed relatedness faster and more cost-effective, but as reliable as PFGE.

Suggested Citation

  • Adrian Egli & Sarah Tschudin-Sutter & Michael Oberle & Daniel Goldenberger & Reno Frei & Andreas F Widmer, 2015. "Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass-Spectrometry (MALDI-TOF MS) Based Typing of Extended-Spectrum β-Lactamase Producing E. coli – A Novel Tool for Real-Time Outbreak Invest," PLOS ONE, Public Library of Science, vol. 10(4), pages 1-6, April.
    • Handle: RePEc:plo:pone00:0120624
    DOI: 10.1371/journal.pone.0120624
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