Author
Listed:
- Meysam Bastani
- Larissa Vos
- Nasimeh Asgarian
- Jean Deschenes
- Kathryn Graham
- John Mackey
- Russell Greiner
Abstract
Background: Selecting the appropriate treatment for breast cancer requires accurately determining the estrogen receptor (ER) status of the tumor. However, the standard for determining this status, immunohistochemical analysis of formalin-fixed paraffin embedded samples, suffers from numerous technical and reproducibility issues. Assessment of ER-status based on RNA expression can provide more objective, quantitative and reproducible test results. Methods: To learn a parsimonious RNA-based classifier of hormone receptor status, we applied a machine learning tool to a training dataset of gene expression microarray data obtained from 176 frozen breast tumors, whose ER-status was determined by applying ASCO-CAP guidelines to standardized immunohistochemical testing of formalin fixed tumor. Results: This produced a three-gene classifier that can predict the ER-status of a novel tumor, with a cross-validation accuracy of 93.17±2.44%. When applied to an independent validation set and to four other public databases, some on different platforms, this classifier obtained over 90% accuracy in each. In addition, we found that this prediction rule separated the patients' recurrence-free survival curves with a hazard ratio lower than the one based on the IHC analysis of ER-status. Conclusions: Our efficient and parsimonious classifier lends itself to high throughput, highly accurate and low-cost RNA-based assessments of ER-status, suitable for routine high-throughput clinical use. This analytic method provides a proof-of-principle that may be applicable to developing effective RNA-based tests for other biomarkers and conditions.
Suggested Citation
Meysam Bastani & Larissa Vos & Nasimeh Asgarian & Jean Deschenes & Kathryn Graham & John Mackey & Russell Greiner, 2013.
"A Machine Learned Classifier That Uses Gene Expression Data to Accurately Predict Estrogen Receptor Status,"
PLOS ONE, Public Library of Science, vol. 8(12), pages 1-9, December.
Handle:
RePEc:plo:pone00:0082144
DOI: 10.1371/journal.pone.0082144
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