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Insulin Receptor Isoforms A and B as well as Insulin Receptor Substrates-1 and -2 Are Differentially Expressed in Prostate Cancer

Author

Listed:
  • Martin Heni
  • Jörg Hennenlotter
  • Marcus Scharpf
  • Stefan Z Lutz
  • Christian Schwentner
  • Tilman Todenhöfer
  • David Schilling
  • Ursula Kühs
  • Valentina Gerber
  • Fausto Machicao
  • Harald Staiger
  • Hans-Ulrich Häring
  • Arnulf Stenzl

Abstract

Aims/Hypothesis: In different cancers types, insulin receptor isoform composition or insulin receptor substrate (IRS) isoforms are different to healthy tissue. This may be a molecular link to increased cancer risk in diabetes and obesity. Since this is yet unclear for prostate cancer, we investigated IR isoform composition and IRS balance in prostate cancer compared to benign and tumor adjacent benign prostate tissue and brought this into relation to cell proliferation. Methods: We studied 23 benign prostate samples from radical cystectomy or benign prostatic hyperplasia surgery, 30 samples from benign tissue directly adjacent to prostate cancer foci and 35 cancer samples from different patients. RNA expression levels for insulin receptor isoforms A and B, IRS-1, IRS-2, and IGF-1 receptor were assessed by quantitative real-time RT-PCR. In addition, RNA- and protein expression of the cell cycle regulator p27Kip1 was quantified by real-time RT-PCR and immunohistochemistry. Results: Insulin receptor isoform A to B ratio was significantly higher in cancer as well as in tumor adjacent benign prostate tissue compared to purely benign prostates (p

Suggested Citation

  • Martin Heni & Jörg Hennenlotter & Marcus Scharpf & Stefan Z Lutz & Christian Schwentner & Tilman Todenhöfer & David Schilling & Ursula Kühs & Valentina Gerber & Fausto Machicao & Harald Staiger & Hans, 2012. "Insulin Receptor Isoforms A and B as well as Insulin Receptor Substrates-1 and -2 Are Differentially Expressed in Prostate Cancer," PLOS ONE, Public Library of Science, vol. 7(12), pages 1-6, December.
    • Handle: RePEc:plo:pone00:0050953
    DOI: 10.1371/journal.pone.0050953
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