IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0013917.html
   My bibliography  Save this article

Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers

Author

Listed:
  • Yu-Wei Chiu
  • Men Tzung Lo
  • Ming-Rung Tsai
  • Yi-Chung Chang
  • Rong-Bin Hsu
  • Hsu-Yu Yu
  • Chi-Kuang Sun
  • Yi-Lwun Ho

Abstract

Background: Atrial fibrosis creates a vulnerable tissue for atrial fibrillation (AF), but the spatial disarray of collagen fibers underlying atrial fibrosis is not fully elucidated. Objective: This study hypothesizes that harmonics optical microscopy can illuminate the spatial mal-alignment of collagen fibers in AF via a layer-by-layer approach. Patients and Methods: Atrial tissues taken from patients who underwent open-heart surgery were examined by harmonics optical microscopy. Using the two-dimensional Fourier transformation method, a spectral-energy description of image texture was constituted and its entropy was used to quantify the mal-alignment of collagen fibers. The amount of collagen fiber was derived from its area ratio to total atrial tissue in each image. Serum C-terminal pro-collagen pro-peptide (CICP), pro-matrix metalloproteinase-1 (pro-MMP-1), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) were also evaluated. Results: 46 patients were evaluated, including 20 with normal sinus rhythm and 26 with AF. The entropy of spectral-energy distribution of collagen alignment was significantly higher in AF than that in sinus rhythm (3.97±0.33 vs. 2.80±0.18, p

Suggested Citation

  • Yu-Wei Chiu & Men Tzung Lo & Ming-Rung Tsai & Yi-Chung Chang & Rong-Bin Hsu & Hsu-Yu Yu & Chi-Kuang Sun & Yi-Lwun Ho, 2010. "Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers," PLOS ONE, Public Library of Science, vol. 5(11), pages 1-9, November.
    • Handle: RePEc:plo:pone00:0013917
    DOI: 10.1371/journal.pone.0013917
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013917
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0013917&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0013917?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0013917. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.