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The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

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  • Narumi Ogonuki
  • Manami Mori
  • Akie Shinmen
  • Kimiko Inoue
  • Keiji Mochida
  • Akihiko Ohta
  • Atsuo Ogura

Abstract

Background: Intracytoplasmic sperm injection (ICSI) has been widely used to study the mechanisms of mammalian fertilization and to rescue male-factor infertility in humans and animals. However, very few systematic analyses have been conducted to define factors affecting the efficiency of ICSI. In this study, we undertook a large-scale series of ICSI experiments in mice to define the factors that might affect outcomes. Methodology/Principal Findings: We used a 5×3×2 factorial design with the following factors: mouse genotype (ICR, C57BL/6, DBA/2, C3H/He, and 129/Sv strains), type of male germ cells (epididymal sperm, elongated or round spermatids), and their freeze–thawing treatment. The efficiencies (parameters) of each developmental step were analyzed by three-way ANOVA (significance level P

Suggested Citation

  • Narumi Ogonuki & Manami Mori & Akie Shinmen & Kimiko Inoue & Keiji Mochida & Akihiko Ohta & Atsuo Ogura, 2010. "The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice," PLOS ONE, Public Library of Science, vol. 5(6), pages 1-7, June.
    • Handle: RePEc:plo:pone00:0011062
    DOI: 10.1371/journal.pone.0011062
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