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Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes

Author

Listed:
  • Galina Denisova
  • Dimitri Denisov
  • Carole Evelegh
  • Michaela Weissgram
  • Jochen Beck
  • Stephen Ronan Foley
  • Jonathan Lorne Bramson

Abstract

Background: Antigen-based (as opposed to whole organism) vaccines are actively being pursued for numerous indications. Even though different formulations may produce similar levels of total antigen-specific antibody, the composition of the antibody response can be quite distinct resulting in different levels of therapeutic activity. Methodology/Principal Findings: Using plasmid-based immunization against the proto-oncogene HER-2 as a model, we have demonstrated that affinity-selected epitope mimetics (mimotopes) can provide a defined signature of a polyclonal antibody response. Further, using novel computer algorithms that we have developed, these mimotopes can be used to predict epitope targets. Conclusions/Significance: By combining our novel strategy with existing methods of epitope prediction based on physical properties of an individual protein, we believe that this method offers a robust method for characterizing the breadth of epitope-specificity within a specific polyserum. This strategy is useful as a tool for monitoring immunity following vaccination and can also be used to define relevant epitopes for the creation of novel vaccines.

Suggested Citation

  • Galina Denisova & Dimitri Denisov & Carole Evelegh & Michaela Weissgram & Jochen Beck & Stephen Ronan Foley & Jonathan Lorne Bramson, 2009. "Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes," PLOS ONE, Public Library of Science, vol. 4(4), pages 1-11, April.
    • Handle: RePEc:plo:pone00:0005309
    DOI: 10.1371/journal.pone.0005309
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