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Common Variants of Inflammatory Cytokine Genes Are Associated with Risk of Nephropathy in Type 2 Diabetes among Asian Indians

Author

Listed:
  • Tarunveer Singh Ahluwalia
  • Madhu Khullar
  • Monica Ahuja
  • Harbir Singh Kohli
  • Anil Bhansali
  • Viswanathan Mohan
  • Radha Venkatesan
  • Taranjit Singh Rai
  • Kamal Sud
  • Pawan K Singal

Abstract

Background: Inflammatory cytokine genes have been proposed as good candidate genes for conferring susceptibility to diabetic nephropathy. In the present study, we examined the combined effect of multiple alleles of pro inflammatory cytokine genes for determining the risk of nephropathy in type 2 diabetic patients. Methodology/Principal Findings: Eight single nucleotide polymorphisms (SNPs) of pro-inflammatory cytokine genes (CCL2, TGFB1, IL8, CCR5, and MMP9) were genotyped in two independently ascertained type 2 diabetic cohorts with (DN) and without nephropathy (DM); consisting of patients from North India (n = 495) and South India (n = 188). Genotyping was carried out using PCR, allele specific oligonucleotide-PCR (ASO-PCR), PCR-RFLP and TaqMan allelic discrimination assays and the gene–gene interaction among genetic variants were determined by multi dimensional reduction (MDR) software. Serum high sensitive CRP (hs-CRP) levels were measured by ELISA. The hs-CRP levels were significantly higher in DN as compared to the DM group (p

Suggested Citation

  • Tarunveer Singh Ahluwalia & Madhu Khullar & Monica Ahuja & Harbir Singh Kohli & Anil Bhansali & Viswanathan Mohan & Radha Venkatesan & Taranjit Singh Rai & Kamal Sud & Pawan K Singal, 2009. "Common Variants of Inflammatory Cytokine Genes Are Associated with Risk of Nephropathy in Type 2 Diabetes among Asian Indians," PLOS ONE, Public Library of Science, vol. 4(4), pages 1-10, April.
    • Handle: RePEc:plo:pone00:0005168
    DOI: 10.1371/journal.pone.0005168
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