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A Role for the Juxtamembrane Cytoplasm in the Molecular Dynamics of Focal Adhesions

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  • Haguy Wolfenson
  • Ariel Lubelski
  • Tamar Regev
  • Joseph Klafter
  • Yoav I Henis
  • Benjamin Geiger

Abstract

Focal adhesions (FAs) are specialized membrane-associated multi-protein complexes that link the cell to the extracellular matrix and play crucial roles in cell-matrix sensing. Considerable information is available on the complex molecular composition of these sites, yet the regulation of FA dynamics is largely unknown. Based on a combination of FRAP studies in live cells, with in silico simulations and mathematical modeling, we show that the FA plaque proteins paxillin and vinculin exist in four dynamic states: an immobile FA-bound fraction, an FA-associated fraction undergoing exchange, a juxtamembrane fraction experiencing attenuated diffusion, and a fast-diffusing cytoplasmic pool. The juxtamembrane region surrounding FAs displays a gradient of FA plaque proteins with respect to both concentration and dynamics. Based on these findings, we propose a new model for the regulation of FA dynamics in which this juxtamembrane domain acts as an intermediary layer, enabling an efficient regulation of FA formation and reorganization.

Suggested Citation

  • Haguy Wolfenson & Ariel Lubelski & Tamar Regev & Joseph Klafter & Yoav I Henis & Benjamin Geiger, 2009. "A Role for the Juxtamembrane Cytoplasm in the Molecular Dynamics of Focal Adhesions," PLOS ONE, Public Library of Science, vol. 4(1), pages 1-12, January.
    • Handle: RePEc:plo:pone00:0004304
    DOI: 10.1371/journal.pone.0004304
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