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Protein synthesis by ribosomes with tethered subunits

Author

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  • Cédric Orelle

    (Center for Pharmaceutical Biotechnology – m/c 870, University of Illinois at Chicago
    †Present address: Institut de Biologie et Chimie des Protéines, UMR5086 CNRS/Université Lyon 1, 7 passage du Vercors, 69367 Lyon, France.)

  • Erik D. Carlson

    (Northwestern University
    Chemistry of Life Processes Institute, Northwestern University)

  • Teresa Szal

    (Center for Pharmaceutical Biotechnology – m/c 870, University of Illinois at Chicago)

  • Tanja Florin

    (Center for Pharmaceutical Biotechnology – m/c 870, University of Illinois at Chicago)

  • Michael C. Jewett

    (Northwestern University
    Chemistry of Life Processes Institute, Northwestern University)

  • Alexander S. Mankin

    (Center for Pharmaceutical Biotechnology – m/c 870, University of Illinois at Chicago)

Abstract

A ribosome with tethered subunits, ‘Ribo-T’, is engineered by making a hybrid RNA composed of ribosomal RNA of large and small subunits; Ribo-T can support cell growth in vivo in the absence of wild-type ribosomes, and is used to establish a fully orthogonal ribosome–mRNA system.

Suggested Citation

  • Cédric Orelle & Erik D. Carlson & Teresa Szal & Tanja Florin & Michael C. Jewett & Alexander S. Mankin, 2015. "Protein synthesis by ribosomes with tethered subunits," Nature, Nature, vol. 524(7563), pages 119-124, August.
    • Handle: RePEc:nat:nature:v:524:y:2015:i:7563:d:10.1038_nature14862
    DOI: 10.1038/nature14862
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    Cited by:

    1. Molly F. Parsons & Matthew F. Allan & Shanshan Li & Tyson R. Shepherd & Sakul Ratanalert & Kaiming Zhang & Krista M. Pullen & Wah Chiu & Silvi Rouskin & Mark Bathe, 2023. "3D RNA-scaffolded wireframe origami," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Minkoo Ahn & Tomasz Włodarski & Alkistis Mitropoulou & Sammy H. S. Chan & Haneesh Sidhu & Elena Plessa & Thomas A. Becker & Nediljko Budisa & Christopher A. Waudby & Roland Beckmann & Anaïs M. E. Cass, 2022. "Modulating co-translational protein folding by rational design and ribosome engineering," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Antje Krüger & Andrew M. Watkins & Roger Wellington-Oguri & Jonathan Romano & Camila Kofman & Alysse DeFoe & Yejun Kim & Jeff Anderson-Lee & Eli Fisker & Jill Townley & Anne E. d’Aquino & Rhiju Das & , 2023. "Community science designed ribosomes with beneficial phenotypes," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. Roberto Di Blasi & Mara Pisani & Fabiana Tedeschi & Masue M. Marbiah & Karen Polizzi & Simone Furini & Velia Siciliano & Francesca Ceroni, 2023. "Resource-aware construct design in mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    5. Felix Gersteuer & Martino Morici & Sara Gabrielli & Keigo Fujiwara & Haaris A. Safdari & Helge Paternoga & Lars V. Bock & Shinobu Chiba & Daniel N. Wilson, 2024. "The SecM arrest peptide traps a pre-peptide bond formation state of the ribosome," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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