IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v472y2011i7344d10.1038_nature09929.html
   My bibliography  Save this article

A system for the continuous directed evolution of biomolecules

Author

Listed:
  • Kevin M. Esvelt

    (Harvard University)

  • Jacob C. Carlson

    (Harvard University)

  • David R. Liu

    (Harvard University
    Howard Hughes Medical Institute)

Abstract

Speedy route to new biomolecules Many biomolecules with useful properties have been generated by laboratory molecular evolution experiments, but the processes typically take days and require frequent human intervention. Esvelt et al. now describe a phage-assisted continuous evolution system that enables the continuous, directed evolution of gene-encoded molecules that can be linked to protein production in Escherichia coli. Dozens of rounds of evolution can occur in a single day using this method, as demonstrated by the evolution of novel types of T7 RNA polymerase.

Suggested Citation

  • Kevin M. Esvelt & Jacob C. Carlson & David R. Liu, 2011. "A system for the continuous directed evolution of biomolecules," Nature, Nature, vol. 472(7344), pages 499-503, April.
    • Handle: RePEc:nat:nature:v:472:y:2011:i:7344:d:10.1038_nature09929
    DOI: 10.1038/nature09929
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature09929
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature09929?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Lara Sellés Vidal & James W. Murray & John T. Heap, 2021. "Versatile selective evolutionary pressure using synthetic defect in universal metabolism," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    2. Shuke Wu & Chao Xiang & Yi Zhou & Mohammad Saiful Hasan Khan & Weidong Liu & Christian G. Feiler & Ren Wei & Gert Weber & Matthias Höhne & Uwe T. Bornscheuer, 2022. "A growth selection system for the directed evolution of amine-forming or converting enzymes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    3. Anna Zimmermann & Julian E. Prieto-Vivas & Charlotte Cautereels & Anton Gorkovskiy & Jan Steensels & Yves Peer & Kevin J. Verstrepen, 2023. "A Cas3-base editing tool for targetable in vivo mutagenesis," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Enrico Orsi & Lennart Schada von Borzyskowski & Stephan Noack & Pablo I. Nikel & Steffen N. Lindner, 2024. "Automated in vivo enzyme engineering accelerates biocatalyst optimization," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    5. Simeon D. Castle & Michiel Stock & Thomas E. Gorochowski, 2024. "Engineering is evolution: a perspective on design processes to engineer biology," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    6. Jeonghye Yu & Jongpil Shin & Jihwan Yu & Jihye Kim & Daseuli Yu & Won Do Heo, 2024. "Programmable RNA base editing with photoactivatable CRISPR-Cas13," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    7. Yanik Weber & Desirée Böck & Anastasia Ivașcu & Nicolas Mathis & Tanja Rothgangl & Eleonora I. Ioannidi & Alex C. Blaudt & Lisa Tidecks & Máté Vadovics & Hiromi Muramatsu & Andreas Reichmuth & Kim F. , 2024. "Enhancing prime editor activity by directed protein evolution in yeast," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    8. Emily Zhang & Monica E. Neugebauer & Nicholas A. Krasnow & David R. Liu, 2024. "Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    9. Mary S. Morrison & Tina Wang & Aditya Raguram & Colin Hemez & David R. Liu, 2021. "Disulfide-compatible phage-assisted continuous evolution in the periplasmic space," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    10. Stefan A Hoffmann & Christian Wohltat & Kristian M Müller & Katja M Arndt, 2017. "A user-friendly, low-cost turbidostat with versatile growth rate estimation based on an extended Kalman filter," PLOS ONE, Public Library of Science, vol. 12(7), pages 1-15, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:472:y:2011:i:7344:d:10.1038_nature09929. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.