IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v468y2010i7324d10.1038_nature09479.html
   My bibliography  Save this article

U1 snRNP protects pre-mRNAs from premature cleavage and polyadenylation

Author

Listed:
  • Daisuke Kaida

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Michael G. Berg

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Ihab Younis

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Mumtaz Kasim

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Larry N. Singh

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Lili Wan

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

  • Gideon Dreyfuss

    (Howard Hughes Medical Institute, University of Pennsylvania School of Medicine)

Abstract

In eukaryotes, U1 small nuclear ribonucleoprotein (snRNP) forms spliceosomes in equal stoichiometry with U2, U4, U5 and U6 snRNPs; however, its abundance in human far exceeds that of the other snRNPs. Here we used antisense morpholino oligonucleotide to U1 snRNA to achieve functional U1 snRNP knockdown in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays. In addition to inhibiting splicing, U1 snRNP knockdown caused premature cleavage and polyadenylation in numerous pre-mRNAs at cryptic polyadenylation signals, frequently in introns near (

Suggested Citation

  • Daisuke Kaida & Michael G. Berg & Ihab Younis & Mumtaz Kasim & Larry N. Singh & Lili Wan & Gideon Dreyfuss, 2010. "U1 snRNP protects pre-mRNAs from premature cleavage and polyadenylation," Nature, Nature, vol. 468(7324), pages 664-668, December.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7324:d:10.1038_nature09479
    DOI: 10.1038/nature09479
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature09479
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature09479?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Emily Kunce Stroup & Zhe Ji, 2023. "Deep learning of human polyadenylation sites at nucleotide resolution reveals molecular determinants of site usage and relevance in disease," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Amy L. Hughes & Aleksander T. Szczurek & Jessica R. Kelley & Anna Lastuvkova & Anne H. Turberfield & Emilia Dimitrova & Neil P. Blackledge & Robert J. Klose, 2023. "A CpG island-encoded mechanism protects genes from premature transcription termination," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:468:y:2010:i:7324:d:10.1038_nature09479. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.