IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v468y2010i7321d10.1038_nature09531.html
   My bibliography  Save this article

A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity

Author

Listed:
  • Na-Yu Chia

    (Gene Regulation Laboratory, Genome Institute of Singapore
    School of Biological Sciences, Nanyang Technological University)

  • Yun-Shen Chan

    (Gene Regulation Laboratory, Genome Institute of Singapore
    Graduate School for Integrative Sciences & Engineering, National University of Singapore)

  • Bo Feng

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Xinyi Lu

    (Gene Regulation Laboratory, Genome Institute of Singapore
    Dept of Biological Sciences, National University of Singapore)

  • Yuriy L. Orlov

    (Computational and Systems Biology group, Genome Institute of Singapore)

  • Dimitri Moreau

    (Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673)

  • Pankaj Kumar

    (Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673)

  • Lin Yang

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Jianming Jiang

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Mei-Sheng Lau

    (Gene Regulation Laboratory, Genome Institute of Singapore)

  • Mikael Huss

    (Computational and Systems Biology group, Genome Institute of Singapore)

  • Boon-Seng Soh

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Petra Kraus

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Pin Li

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Thomas Lufkin

    (Stem Cell and Developmental Biology, Genome Institute of Singapore)

  • Bing Lim

    (Stem Cell and Developmental Biology, Genome Institute of Singapore
    Center for Life Sciences, Harvard Medical School, 330 Brookline Avenue, Boston, Massachusetts 02115, USA)

  • Neil D. Clarke

    (Computational and Systems Biology group, Genome Institute of Singapore
    National University of Singapore, 8 Medical Drive, Yong Loo Lin School of Medicine)

  • Frederic Bard

    (Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673
    National University of Singapore, 8 Medical Drive, Yong Loo Lin School of Medicine)

  • Huck-Hui Ng

    (Gene Regulation Laboratory, Genome Institute of Singapore
    School of Biological Sciences, Nanyang Technological University
    Graduate School for Integrative Sciences & Engineering, National University of Singapore
    Dept of Biological Sciences, National University of Singapore)

Abstract

Screen test for stem-cell cues If the full potential of human embryonic stem (ES) cells in research and clinical applications is to be realized, we need a detailed understanding of the genetic network that governs their unique properties. A genome-wide RNA interference screen identifies a wealth of regulators of self-renewal and pluripotency properties in human embryonic stem cells. PRDM14, for example, represents a key transcription factor required for the maintenance of human ES cell identity and reprogramming of somatic cells to pluripotency.

Suggested Citation

  • Na-Yu Chia & Yun-Shen Chan & Bo Feng & Xinyi Lu & Yuriy L. Orlov & Dimitri Moreau & Pankaj Kumar & Lin Yang & Jianming Jiang & Mei-Sheng Lau & Mikael Huss & Boon-Seng Soh & Petra Kraus & Pin Li & Thom, 2010. "A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity," Nature, Nature, vol. 468(7321), pages 316-320, November.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7321:d:10.1038_nature09531
    DOI: 10.1038/nature09531
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature09531
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature09531?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Quentin I. B. Lemaître & Natascha Bartsch & Ian U. Kouzel & Henriette Busengdal & Gemma Sian Richards & Patrick R. H. Steinmetz & Fabian Rentzsch, 2023. "NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:468:y:2010:i:7321:d:10.1038_nature09531. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.