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The CRISPR tool kit for genome editing and beyond

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  • Mazhar Adli

    (University of Virginia)

Abstract

CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, and agriculture. CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering, and imaging, now exceed the gene-editing functionality of WT Cas9. Here, we will present a brief history of gene-editing tools and describe the wide range of CRISPR-based genome-targeting tools. We will conclude with future directions and the broader impact of CRISPR technologies.

Suggested Citation

  • Mazhar Adli, 2018. "The CRISPR tool kit for genome editing and beyond," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04252-2
    DOI: 10.1038/s41467-018-04252-2
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    Cited by:

    1. Jiongyu Zhang & Chengyu Hou & Changchun Liu, 2024. "CRISPR-powered quantitative keyword search engine in DNA data storage," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Yekaterina S Pavlova & David Paez-Espino & Andrew Yu Morozov & Ilya S Belalov, 2021. "Searching for fat tails in CRISPR-Cas systems: Data analysis and mathematical modeling," PLOS Computational Biology, Public Library of Science, vol. 17(3), pages 1-21, March.
    3. Aaron A. Smargon & Assael A. Madrigal & Brian A. Yee & Kevin D. Dong & Jasmine R. Mueller & Gene W. Yeo, 2022. "Crosstalk between CRISPR-Cas9 and the human transcriptome," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    4. Dalton T. Ham & Tyler S. Browne & Pooja N. Banglorewala & Tyler L. Wilson & Richard K. Michael & Gregory B. Gloor & David R. Edgell, 2023. "A generalizable Cas9/sgRNA prediction model using machine transfer learning with small high-quality datasets," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    5. Nic Robertson & Vadim Shchepachev & David Wright & Tomasz W. Turowski & Christos Spanos & Aleksandra Helwak & Rose Zamoyska & David Tollervey, 2022. "A disease-linked lncRNA mutation in RNase MRP inhibits ribosome synthesis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    6. Sehrish Khan & Muhammad Shahid Mahmood & Sajjad ur Rahman & Farzana Rizvi & Aftab Ahmad, 2020. "Evaluation of the CRISPR/Cas9 system for the development of resistance against Cotton leaf curl virus in model plants," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 56(3), pages 154-162.
    7. Daniel C. Volke & Román A. Martino & Ekaterina Kozaeva & Andrea M. Smania & Pablo I. Nikel, 2022. "Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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