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TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells

Author

Listed:
  • Shin-Ichiroh Saitoh

    (The Institute of Medical Science, The University of Tokyo)

  • Fumiko Abe

    (The University of Tokyo)

  • Atsuo Kanno

    (The Institute of Medical Science, The University of Tokyo)

  • Natsuko Tanimura

    (The Institute of Medical Science, The University of Tokyo)

  • Yoshiko Mori Saitoh

    (The Institute of Medical Science, The University of Tokyo)

  • Ryutaro Fukui

    (The Institute of Medical Science, The University of Tokyo)

  • Takuma Shibata

    (The Institute of Medical Science, The University of Tokyo)

  • Katsuaki Sato

    (University of Miyazaki)

  • Takeshi Ichinohe

    (The University of Tokyo)

  • Mayumi Hayashi

    (Ltd., 1-16-13 Kitakasai)

  • Kazuishi Kubota

    (Ltd., 1-16-13 Kitakasai)

  • Hiroko Kozuka-Hata

    (The University of Tokyo)

  • Masaaki Oyama

    (The University of Tokyo)

  • Yorifumi Kikko

    (The University of Tokyo)

  • Toshiaki Katada

    (The University of Tokyo)

  • Kenji Kontani

    (The University of Tokyo
    Meiji Pharmaceutical University)

  • Kensuke Miyake

    (The Institute of Medical Science, The University of Tokyo
    The Institute of Medical Science, The University of Tokyo)

Abstract

Plasmacytoid dendritic cells (pDC) sense viral RNA through toll-like receptor 7 (TLR7), form self-adhesive pDC–pDC clusters, and produce type I interferons. This cell adhesion enhances type I interferon production, but little is known about the underlying mechanisms. Here we show that MyD88-dependent TLR7 signaling activates CD11a/CD18 integrin to induce microtubule elongation. TLR7+ lysosomes then become linked with these microtubules through the GTPase Arl8b and its effector SKIP/Plekhm2, resulting in perinuclear to peripheral relocalization of TLR7. The type I interferon signaling molecules TRAF3, IKKα, and mTORC1 are constitutively associated in pDCs. TLR7 localizes to mTORC1 and induces association of TRAF3 with the upstream molecule TRAF6. Finally, type I interferons are secreted in the vicinity of cell–cell contacts between clustered pDCs. These results suggest that TLR7 needs to move to the cell periphery to induce robust type I interferon responses in pDCs.

Suggested Citation

  • Shin-Ichiroh Saitoh & Fumiko Abe & Atsuo Kanno & Natsuko Tanimura & Yoshiko Mori Saitoh & Ryutaro Fukui & Takuma Shibata & Katsuaki Sato & Takeshi Ichinohe & Mayumi Hayashi & Kazuishi Kubota & Hiroko , 2017. "TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01687-x
    DOI: 10.1038/s41467-017-01687-x
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    Cited by:

    1. Manon Venet & Margarida Sa Ribeiro & Elodie Décembre & Alicia Bellomo & Garima Joshi & Célia Nuovo & Marine Villard & David Cluet & Magali Perret & Rémi Pescamona & Helena Paidassi & Thierry Walzer & , 2023. "Severe COVID-19 patients have impaired plasmacytoid dendritic cell-mediated control of SARS-CoV-2," Nature Communications, Nature, vol. 14(1), pages 1-21, December.

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