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Resolving mixed mechanisms of protein subdiffusion at the T cell plasma membrane

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  • Yonatan Golan

    (Racah Institute of Physics, The Hebrew University)

  • Eilon Sherman

    (Racah Institute of Physics, The Hebrew University)

Abstract

The plasma membrane is a complex medium where transmembrane proteins diffuse and interact to facilitate cell function. Membrane protein mobility is affected by multiple mechanisms, including crowding, trapping, medium elasticity and structure, thus limiting our ability to distinguish them in intact cells. Here we characterize the mobility and organization of a short transmembrane protein at the plasma membrane of live T cells, using single particle tracking and photoactivated-localization microscopy. Protein mobility is highly heterogeneous, subdiffusive and ergodic-like. Using mobility characteristics, we segment individual trajectories into subpopulations with distinct Gaussian step-size distributions. Particles of low-to-medium mobility consist of clusters, diffusing in a viscoelastic and fractal-like medium and are enriched at the centre of the cell footprint. Particles of high mobility undergo weak confinement and are more evenly distributed. This study presents a methodological approach to resolve simultaneous mixed subdiffusion mechanisms acting on polydispersed samples and complex media such as cell membranes.

Suggested Citation

  • Yonatan Golan & Eilon Sherman, 2017. "Resolving mixed mechanisms of protein subdiffusion at the T cell plasma membrane," Nature Communications, Nature, vol. 8(1), pages 1-15, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15851
    DOI: 10.1038/ncomms15851
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    Cited by:

    1. dos Santos, M.A.F. & Menon, L. & Cius, D., 2022. "Superstatistical approach of the anomalous exponent for scaled Brownian motion," Chaos, Solitons & Fractals, Elsevier, vol. 164(C).
    2. Nicky Scheefhals & Manon Westra & Harold D. MacGillavry, 2023. "mGluR5 is transiently confined in perisynaptic nanodomains to shape synaptic function," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    3. Faïçal Ndaïrou & Delfim F. M. Torres, 2021. "Pontryagin Maximum Principle for Distributed-Order Fractional Systems," Mathematics, MDPI, vol. 9(16), pages 1-12, August.

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