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Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells

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Listed:
  • Anthony J. Mangan

    (School of Medicine, Anschutz Medical Campus, University of Colorado Denver)

  • Daniel V. Sietsema

    (School of Medicine, Anschutz Medical Campus, University of Colorado Denver)

  • Dongying Li

    (School of Medicine, Anschutz Medical Campus, University of Colorado Denver)

  • Jeffrey K. Moore

    (School of Medicine, Anschutz Medical Campus, University of Colorado Denver)

  • Sandra Citi

    (University of Geneva)

  • Rytis Prekeris

    (School of Medicine, Anschutz Medical Campus, University of Colorado Denver)

Abstract

Coordinated polarization of epithelial cells is a key step during morphogenesis that leads to the formation of an apical lumen. Rab11 and its interacting protein FIP5 are necessary for the targeting of apical endosomes to the midbody and apical membrane initiation site (AMIS) during lumenogenesis. However, the machinery that mediates AMIS establishment and FIP5-endosome targeting remains unknown. Here we identify a FIP5-interacting protein, Cingulin, which localizes to the AMIS and functions as a tether mediating FIP5-endosome targeting. We analysed the machinery mediating AMIS recruitment to the midbody and determined that both branched actin and microtubules are required for establishing the site of the nascent lumen. We demonstrate that the Rac1-WAVE/Scar complex mediates Cingulin recruitment to the AMIS by inducing branched actin formation, and that Cingulin directly binds to microtubule C-terminal tails through electrostatic interactions. We propose a new mechanism for apical endosome targeting and AMIS formation around the midbody during epithelial lumenogenesis.

Suggested Citation

  • Anthony J. Mangan & Daniel V. Sietsema & Dongying Li & Jeffrey K. Moore & Sandra Citi & Rytis Prekeris, 2016. "Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells," Nature Communications, Nature, vol. 7(1), pages 1-15, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12426
    DOI: 10.1038/ncomms12426
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    Cited by:

    1. Eva C. Freckmann & Emma Sandilands & Erin Cumming & Matthew Neilson & Alvaro Román-Fernández & Konstantina Nikolatou & Marisa Nacke & Tamsin R. M. Lannagan & Ann Hedley & David Strachan & Mark Salji &, 2022. "Traject3d allows label-free identification of distinct co-occurring phenotypes within 3D culture by live imaging," Nature Communications, Nature, vol. 13(1), pages 1-21, December.

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