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Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution

Author

Listed:
  • Lei Sun

    (Purdue University)

  • Xinzheng Zhang

    (Purdue University
    Present addresses: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.)

  • Song Gao

    (The Catholic University of America, 620 Michigan Ave. N.E., Washington, DC 20064, USA
    Present addresses: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China.)

  • Prashant A. Rao

    (National Cancer Institute, National Institutes of Health)

  • Victor Padilla-Sanchez

    (The Catholic University of America, 620 Michigan Ave. N.E., Washington, DC 20064, USA)

  • Zhenguo Chen

    (Purdue University)

  • Siyang Sun

    (Purdue University)

  • Ye Xiang

    (Purdue University
    Present addresses: School of Medicine, Tsinghua University, Haidian, Beijing 100084, China.)

  • Sriram Subramaniam

    (National Cancer Institute, National Institutes of Health)

  • Venigalla B. Rao

    (The Catholic University of America, 620 Michigan Ave. N.E., Washington, DC 20064, USA)

  • Michael G. Rossmann

    (Purdue University)

Abstract

The structure and assembly of bacteriophage T4 has been extensively studied. However, the detailed structure of the portal protein remained unknown. Here we report the structure of the bacteriophage T4 portal assembly, gene product 20 (gp20), determined by cryo-electron microscopy (cryo-EM) to 3.6 Å resolution. In addition, analysis of a 10 Å resolution cryo-EM map of an empty prolate T4 head shows how the dodecameric portal assembly interacts with the capsid protein gp23 at the special pentameric vertex. The gp20 structure also verifies that the portal assembly is required for initiating head assembly, for attachment of the packaging motor, and for participation in DNA packaging. Comparison of the Myoviridae T4 portal structure with the known portal structures of φ29, SPP1 and P22, representing Podo- and Siphoviridae, shows that the portal structure probably dates back to a time when self-replicating microorganisms were being established on Earth.

Suggested Citation

  • Lei Sun & Xinzheng Zhang & Song Gao & Prashant A. Rao & Victor Padilla-Sanchez & Zhenguo Chen & Siyang Sun & Ye Xiang & Sriram Subramaniam & Venigalla B. Rao & Michael G. Rossmann, 2015. "Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution," Nature Communications, Nature, vol. 6(1), pages 1-11, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8548
    DOI: 10.1038/ncomms8548
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    Cited by:

    1. Jingen Zhu & Himanshu Batra & Neeti Ananthaswamy & Marthandan Mahalingam & Pan Tao & Xiaorong Wu & Wenzheng Guo & Andrei Fokine & Venigalla B. Rao, 2023. "Design of bacteriophage T4-based artificial viral vectors for human genome remodeling," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Igor Orlov & Stéphane Roche & Sandrine Brasilès & Natalya Lukoyanova & Marie-Christine Vaney & Paulo Tavares & Elena V. Orlova, 2022. "CryoEM structure and assembly mechanism of a bacterial virus genome gatekeeper," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Xudong Jia & Yuanzhu Gao & Yuxuan Huang & Linjun Sun & Siduo Li & Hongmei Li & Xueqing Zhang & Yinyin Li & Jian He & Wenbi Wu & Harikanth Venkannagari & Kai Yang & Matthew L. Baker & Qinfen Zhang, 2023. "Architecture of the baculovirus nucleocapsid revealed by cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    4. Yang Huang & Hui Sun & Shuzhen Wei & Lanlan Cai & Liqin Liu & Yanan Jiang & Jiabao Xin & Zhenqin Chen & Yuqiong Que & Zhibo Kong & Tingting Li & Hai Yu & Jun Zhang & Ying Gu & Qingbing Zheng & Shaowei, 2023. "Structure and proposed DNA delivery mechanism of a marine roseophage," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    5. Fenglin Li & Chun-Feng David Hou & Ravi K. Lokareddy & Ruoyu Yang & Francesca Forti & Federica Briani & Gino Cingolani, 2023. "High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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