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APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages

Author

Listed:
  • Shraddha Sharma

    (Roswell Park Cancer Institute)

  • Santosh K. Patnaik

    (Roswell Park Cancer Institute)

  • R. Thomas Taggart

    (Roswell Park Cancer Institute)

  • Eric D. Kannisto

    (Roswell Park Cancer Institute)

  • Sally M. Enriquez

    (University at Buffalo, State University of New York)

  • Paul Gollnick

    (University at Buffalo, State University of New York)

  • Bora E. Baysal

    (Roswell Park Cancer Institute)

Abstract

The extent, regulation and enzymatic basis of RNA editing by cytidine deamination are incompletely understood. Here we show that transcripts of hundreds of genes undergo site-specific C>U RNA editing in macrophages during M1 polarization and in monocytes in response to hypoxia and interferons. This editing alters the amino acid sequences for scores of proteins, including many that are involved in pathogenesis of viral diseases. APOBEC3A, which is known to deaminate cytidines of single-stranded DNA and to inhibit viruses and retrotransposons, mediates this RNA editing. Amino acid residues of APOBEC3A that are known to be required for its DNA deamination and anti-retrotransposition activities were also found to affect its RNA deamination activity. Our study demonstrates the cellular RNA editing activity of a member of the APOBEC3 family of innate restriction factors and expands the understanding of C>U RNA editing in mammals.

Suggested Citation

  • Shraddha Sharma & Santosh K. Patnaik & R. Thomas Taggart & Eric D. Kannisto & Sally M. Enriquez & Paul Gollnick & Bora E. Baysal, 2015. "APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages," Nature Communications, Nature, vol. 6(1), pages 1-15, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7881
    DOI: 10.1038/ncomms7881
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    Cited by:

    1. Stefan Harjes & Harikrishnan M. Kurup & Amanda E. Rieffer & Maitsetseg Bayarjargal & Jana Filitcheva & Yongdong Su & Tracy K. Hale & Vyacheslav V. Filichev & Elena Harjes & Reuben S. Harris & Geoffrey, 2023. "Structure-guided inhibition of the cancer DNA-mutating enzyme APOBEC3A," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Yasha Butt & Ramin Sakhtemani & Rukshana Mohamad-Ramshan & Michael S. Lawrence & Ashok S. Bhagwat, 2024. "Distinguishing preferences of human APOBEC3A and APOBEC3B for cytosines in hairpin loops, and reflection of these preferences in APOBEC-signature cancer genome mutations," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Ambrocio Sanchez & Pedro Ortega & Ramin Sakhtemani & Lavanya Manjunath & Sunwoo Oh & Elodie Bournique & Alexandrea Becker & Kyumin Kim & Cameron Durfee & Nuri Alpay Temiz & Xiaojiang S. Chen & Reuben , 2024. "Mesoscale DNA features impact APOBEC3A and APOBEC3B deaminase activity and shape tumor mutational landscapes," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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