IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms6104.html
   My bibliography  Save this article

Visualizing the replication of respiratory syncytial virus in cells and in living mice

Author

Listed:
  • Marie-Anne Rameix-Welti

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA
    Physiopathologie et diagnostic des infections microbiennes, EA3647—EPIM, UFR des Sciences de la Santé Simone Veil—UVSQ
    AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie)

  • Ronan Le Goffic

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA)

  • Pierre-Louis Hervé

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA)

  • Julien Sourimant

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA
    Physiopathologie et diagnostic des infections microbiennes, EA3647—EPIM, UFR des Sciences de la Santé Simone Veil—UVSQ)

  • Aude Rémot

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA)

  • Sabine Riffault

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA)

  • Qin Yu

    (Infection Innovative Medicines Unit, AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, Massachusetts 02451, USA)

  • Marie Galloux

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA)

  • Elyanne Gault

    (Physiopathologie et diagnostic des infections microbiennes, EA3647—EPIM, UFR des Sciences de la Santé Simone Veil—UVSQ
    AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie)

  • Jean-François Eléouët

    (Unité de Virologie et Immunologie Moleculaires (UR892), INRA)

Abstract

Respiratory syncytial virus (RSV) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. Expression of mCherry and Luc are correlated with infection rate, allowing the monitoring of RSV multiplication in cell culture. Replication of the Luc-encoding virus in living mice can be visualized by bioluminescent imaging, bioluminescence being detected in the snout and lungs of infected mice after nasal inoculation. We propose that these recombinant viruses are convenient and valuable tools for screening of compounds active against RSV, and can be used as an extremely sensitive readout for studying effects of antiviral therapeutics in living mice.

Suggested Citation

  • Marie-Anne Rameix-Welti & Ronan Le Goffic & Pierre-Louis Hervé & Julien Sourimant & Aude Rémot & Sabine Riffault & Qin Yu & Marie Galloux & Elyanne Gault & Jean-François Eléouët, 2014. "Visualizing the replication of respiratory syncytial virus in cells and in living mice," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6104
    DOI: 10.1038/ncomms6104
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms6104
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms6104?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6104. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.