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Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells

Author

Listed:
  • Shin-Ichi Mae

    (Center for iPS Cell Research and Application, Kyoto University)

  • Akemi Shono

    (Center for iPS Cell Research and Application, Kyoto University)

  • Fumihiko Shiota

    (Center for iPS Cell Research and Application, Kyoto University)

  • Tetsuhiko Yasuno

    (Center for iPS Cell Research and Application, Kyoto University)

  • Masatoshi Kajiwara

    (Center for iPS Cell Research and Application, Kyoto University)

  • Nanaka Gotoda-Nishimura

    (Center for iPS Cell Research and Application, Kyoto University)

  • Sayaka Arai

    (Center for iPS Cell Research and Application, Kyoto University)

  • Aiko Sato-Otubo

    (Cancer Genomics Project, The University of Tokyo)

  • Taro Toyoda

    (Center for iPS Cell Research and Application, Kyoto University)

  • Kazutoshi Takahashi

    (Center for iPS Cell Research and Application, Kyoto University)

  • Naoki Nakayama

    (Centre for Stem Cell Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston)

  • Chad A. Cowan

    (Harvard Stem Cell Institute
    Harvard University)

  • Takashi Aoi

    (Center for iPS Cell Research and Application, Kyoto University)

  • Seishi Ogawa

    (Cancer Genomics Project, The University of Tokyo)

  • Andrew P. McMahon

    (Eli and Edythe Broad-Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern California)

  • Shinya Yamanaka

    (Center for iPS Cell Research and Application, Kyoto University
    Institute for Integrated Cell–Material Sciences, Kyoto University
    JST Yamanaka iPS Cell Special Project, Japan Science and Technology Agency
    Gladstone Institute of Cardiovascular Disease)

  • Kenji Osafune

    (Center for iPS Cell Research and Application, Kyoto University
    JST Yamanaka iPS Cell Special Project, Japan Science and Technology Agency
    PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan)

Abstract

A method for stimulating the differentiation of human pluripotent stem cells into kidney lineages remains to be developed. Most cells in kidney are derived from an embryonic germ layer known as intermediate mesoderm. Here we show the establishment of an efficient system of homologous recombination in human pluripotent stem cells by means of bacterial artificial chromosome-based vectors and single-nucleotide polymorphism array-based detection. This system allowed us to generate human-induced pluripotent stem cell lines containing green fluorescence protein knocked into OSR1, a specific intermediate mesoderm marker. We have also established a robust induction protocol for intermediate mesoderm, which produces up to 90% OSR1+ cells. These human intermediate mesoderm cells can differentiate into multiple cell types of intermediate mesoderm-derived organs in vitro and in vivo, thereby supplying a useful system to elucidate the mechanisms of intermediate mesoderm development and potentially providing a cell source for regenerative therapies of the kidney.

Suggested Citation

  • Shin-Ichi Mae & Akemi Shono & Fumihiko Shiota & Tetsuhiko Yasuno & Masatoshi Kajiwara & Nanaka Gotoda-Nishimura & Sayaka Arai & Aiko Sato-Otubo & Taro Toyoda & Kazutoshi Takahashi & Naoki Nakayama & C, 2013. "Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells," Nature Communications, Nature, vol. 4(1), pages 1-11, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2378
    DOI: 10.1038/ncomms2378
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    Cited by:

    1. Jessica M. Vanslambrouck & Sean B. Wilson & Ker Sin Tan & Ella Groenewegen & Rajeev Rudraraju & Jessica Neil & Kynan T. Lawlor & Sophia Mah & Michelle Scurr & Sara E. Howden & Kanta Subbarao & Melissa, 2022. "Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids," Nature Communications, Nature, vol. 13(1), pages 1-23, December.

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