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ComplexEye: a multi-lens array microscope for high-throughput embedded immune cell migration analysis

Author

Listed:
  • Zülal Cibir

    (University Hospital, University of Duisburg-Essen)

  • Jacqueline Hassel

    (University of Duisburg-Essen)

  • Justin Sonneck

    (Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
    Ruhr-Universität Bochum)

  • Lennart Kowitz

    (Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.)

  • Alexander Beer

    (University Hospital, University of Duisburg-Essen)

  • Andreas Kraus

    (University Hospital, University of Duisburg-Essen)

  • Gabriel Hallekamp

    (University of Duisburg-Essen)

  • Martin Rosenkranz

    (University of Duisburg-Essen)

  • Pascal Raffelberg

    (University of Duisburg-Essen)

  • Sven Olfen

    (University of Duisburg-Essen)

  • Kamil Smilowski

    (University of Duisburg-Essen)

  • Roman Burkard

    (University of Duisburg-Essen)

  • Iris Helfrich

    (Medical Faculty of the Ludwig Maximilian University of Munich)

  • Ali Ata Tuz

    (University Hospital, University of Duisburg-Essen)

  • Vikramjeet Singh

    (University Hospital, University of Duisburg-Essen)

  • Susmita Ghosh

    (Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.)

  • Albert Sickmann

    (Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
    Ruhr-Universität Bochum
    University of Aberdeen)

  • Anne-Kathrin Klebl

    (Lead Discovery Center GmbH)

  • Jan Eike Eickhoff

    (Lead Discovery Center GmbH)

  • Bert Klebl

    (Lead Discovery Center GmbH)

  • Karsten Seidl

    (University of Duisburg-Essen)

  • Jianxu Chen

    (Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.)

  • Anton Grabmaier

    (University of Duisburg-Essen)

  • Reinhard Viga

    (University of Duisburg-Essen)

  • Matthias Gunzer

    (University Hospital, University of Duisburg-Essen
    Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.)

Abstract

Autonomous migration is essential for the function of immune cells such as neutrophils and plays an important role in numerous diseases. The ability to routinely measure or target it would offer a wealth of clinical applications. Video microscopy of live cells is ideal for migration analysis, but cannot be performed at sufficiently high-throughput (HT). Here we introduce ComplexEye, an array microscope with 16 independent aberration-corrected glass lenses spaced at the pitch of a 96-well plate to produce high-resolution movies of migrating cells. With the system, we enable HT migration analysis of immune cells in 96- and 384-well plates with very energy-efficient performance. We demonstrate that the system can measure multiple clinical samples simultaneously. Furthermore, we screen 1000 compounds and identify 17 modifiers of migration in human neutrophils in just 4 days, a task that requires 60-times longer with a conventional video microscope. ComplexEye thus opens the field of phenotypic HT migration screens and enables routine migration analysis for the clinical setting.

Suggested Citation

  • Zülal Cibir & Jacqueline Hassel & Justin Sonneck & Lennart Kowitz & Alexander Beer & Andreas Kraus & Gabriel Hallekamp & Martin Rosenkranz & Pascal Raffelberg & Sven Olfen & Kamil Smilowski & Roman Bu, 2023. "ComplexEye: a multi-lens array microscope for high-throughput embedded immune cell migration analysis," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43765-3
    DOI: 10.1038/s41467-023-43765-3
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    References listed on IDEAS

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    1. Anne Reversat & Florian Gaertner & Jack Merrin & Julian Stopp & Saren Tasciyan & Juan Aguilera & Ingrid Vries & Robert Hauschild & Miroslav Hons & Matthieu Piel & Andrew Callan-Jones & Raphael Voituri, 2020. "Cellular locomotion using environmental topography," Nature, Nature, vol. 582(7813), pages 582-585, June.
    2. Simon F. Merz & Sebastian Korste & Lea Bornemann & Lars Michel & Pia Stock & Anthony Squire & Camille Soun & Daniel R. Engel & Julia Detzer & Holger Lörchner & Dirk M. Hermann & Markus Kamler & Joachi, 2019. "Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    3. Simon F. Merz & Sebastian Korste & Lea Bornemann & Lars Michel & Pia Stock & Anthony Squire & Camille Soun & Daniel R. Engel & Julia Detzer & Holger Lörchner & Dirk M. Hermann & Markus Kamler & Joachi, 2019. "Publisher Correction: Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response," Nature Communications, Nature, vol. 10(1), pages 1-1, December.
    4. Young Min Song & Yizhu Xie & Viktor Malyarchuk & Jianliang Xiao & Inhwa Jung & Ki-Joong Choi & Zhuangjian Liu & Hyunsung Park & Chaofeng Lu & Rak-Hwan Kim & Rui Li & Kenneth B. Crozier & Yonggang Huan, 2013. "Digital cameras with designs inspired by the arthropod eye," Nature, Nature, vol. 497(7447), pages 95-99, May.
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