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A combinatorial code of neurexin-3 alternative splicing controls inhibitory synapses via a trans-synaptic dystroglycan signaling loop

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Listed:
  • Justin H. Trotter

    (Stanford University School of Medicine)

  • Cosmos Yuqi Wang

    (Stanford University School of Medicine)

  • Peng Zhou

    (Stanford University School of Medicine)

  • George Nakahara

    (Stanford University School of Medicine)

  • Thomas C. Südhof

    (Stanford University School of Medicine
    Stanford University School of Medicine)

Abstract

Disrupted synaptic inhibition is implicated in neuropsychiatric disorders, yet the molecular mechanisms that shape and sustain inhibitory synapses are poorly understood. Here, we show through rescue experiments performed using Neurexin-3 conditional knockout mice that alternative splicing at SS2 and SS4 regulates the release probability, but not the number, of inhibitory synapses in the olfactory bulb and prefrontal cortex independent of sex. Neurexin-3 splice variants that mediate Neurexin-3 binding to dystroglycan enable inhibitory synapse function, whereas splice variants that don’t allow dystroglycan binding do not. Furthermore, a minimal Neurexin-3 protein that binds to dystroglycan fully sustains inhibitory synaptic function, indicating that trans-synaptic dystroglycan binding is necessary and sufficient for Neurexin-3 function in inhibitory synaptic transmission. Thus, Neurexin-3 enables a normal release probability at inhibitory synapses via a trans-synaptic feedback signaling loop consisting of presynaptic Neurexin-3 and postsynaptic dystroglycan.

Suggested Citation

  • Justin H. Trotter & Cosmos Yuqi Wang & Peng Zhou & George Nakahara & Thomas C. Südhof, 2023. "A combinatorial code of neurexin-3 alternative splicing controls inhibitory synapses via a trans-synaptic dystroglycan signaling loop," Nature Communications, Nature, vol. 14(1), pages 1-22, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36872-8
    DOI: 10.1038/s41467-023-36872-8
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    References listed on IDEAS

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    1. Markus Missler & Weiqi Zhang & Astrid Rohlmann & Gunnar Kattenstroth & Robert E. Hammer & Kurt Gottmann & Thomas C. Südhof, 2003. "α-Neurexins couple Ca2+ channels to synaptic vesicle exocytosis," Nature, Nature, vol. 423(6943), pages 939-948, June.
    2. Fujun Luo & Alessandra Sclip & Sean Merrill & Thomas C. Südhof, 2021. "Neurexins regulate presynaptic GABAB-receptors at central synapses," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    3. Ed S. Lein & Michael J. Hawrylycz & Nancy Ao & Mikael Ayres & Amy Bensinger & Amy Bernard & Andrew F. Boe & Mark S. Boguski & Kevin S. Brockway & Emi J. Byrnes & Lin Chen & Li Chen & Tsuey-Ming Chen &, 2007. "Genome-wide atlas of gene expression in the adult mouse brain," Nature, Nature, vol. 445(7124), pages 168-176, January.
    4. Jinye Dai & Christopher Patzke & Kif Liakath-Ali & Erica Seigneur & Thomas C. Südhof, 2021. "GluD1 is a signal transduction device disguised as an ionotropic receptor," Nature, Nature, vol. 595(7866), pages 261-265, July.
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    Cited by:

    1. Brian A Lloyd & Ying Han & Rebecca Roth & Bo Zhang & Jason Aoto, 2023. "Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus," Nature Communications, Nature, vol. 14(1), pages 1-22, December.

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