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A paralog of Pcc1 is the fifth core subunit of the KEOPS tRNA-modifying complex in Archaea

Author

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  • Marie-Claire Daugeron

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Sophia Missoury

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
    Institut Pasteur)

  • Violette Cunha

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
    Univ Evry, Université Paris-Saclay)

  • Noureddine Lazar

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Bruno Collinet

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
    Institut de Minéralogie de Physique des Matériaux et de Cosmochimie (IMPMC), Sorbonne-Université, UMR7590 CNRS, MNHN)

  • Herman Tilbeurgh

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Tamara Basta

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

Abstract

In Archaea and Eukaryotes, the synthesis of a universal tRNA modification, N6-threonyl-carbamoyl adenosine (t6A), is catalyzed by the KEOPS complex composed of Kae1, Bud32, Cgi121, and Pcc1. A fifth subunit, Gon7, is found only in Fungi and Metazoa. Here, we identify and characterize a fifth KEOPS subunit in Archaea. This protein, dubbed Pcc2, is a paralog of Pcc1 and is widely conserved in Archaea. Pcc1 and Pcc2 form a heterodimer in solution, and show modest sequence conservation but very high structural similarity. The five-subunit archaeal KEOPS does not form dimers but retains robust tRNA binding and t6A synthetic activity. Pcc2 can substitute for Pcc1 but the resulting KEOPS complex is inactive, suggesting a distinct function for the two paralogs. Comparative sequence and structure analyses point to a possible evolutionary link between archaeal Pcc2 and eukaryotic Gon7. Our work indicates that Pcc2 regulates the oligomeric state of the KEOPS complex, a feature that seems to be conserved from Archaea to Eukaryotes.

Suggested Citation

  • Marie-Claire Daugeron & Sophia Missoury & Violette Cunha & Noureddine Lazar & Bruno Collinet & Herman Tilbeurgh & Tamara Basta, 2023. "A paralog of Pcc1 is the fifth core subunit of the KEOPS tRNA-modifying complex in Archaea," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36210-y
    DOI: 10.1038/s41467-023-36210-y
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    References listed on IDEAS

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    1. Changyi Zhang & Alex P. R. Phillips & Rebecca L. Wipfler & Gary J. Olsen & Rachel J. Whitaker, 2018. "The essential genome of the crenarchaeal model Sulfolobus islandicus," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    2. Jonah Beenstock & Samara Mishelle Ona & Jennifer Porat & Stephen Orlicky & Leo C. K. Wan & Derek F. Ceccarelli & Pierre Maisonneuve & Rachel K. Szilard & Zhe Yin & Dheva Setiaputra & Daniel Y. L. Mao , 2020. "A substrate binding model for the KEOPS tRNA modifying complex," Nature Communications, Nature, vol. 11(1), pages 1-17, December.
    3. F. Lemoine & J.-B. Domelevo Entfellner & E. Wilkinson & D. Correia & M. Dávila Felipe & T. Oliveira & O. Gascuel, 2018. "Renewing Felsenstein’s phylogenetic bootstrap in the era of big data," Nature, Nature, vol. 556(7702), pages 452-456, April.
    4. Christelle Arrondel & Sophia Missoury & Rozemarijn Snoek & Julie Patat & Giulia Menara & Bruno Collinet & Dominique Liger & Dominique Durand & Olivier Gribouval & Olivia Boyer & Laurine Buscara & Gaël, 2019. "Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
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