IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-34585-y.html
   My bibliography  Save this article

Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre

Author

Listed:
  • Katie J. Harding

    (University of California
    Lawrence Livermore National Laboratory)

  • Kendra A. Turk-Kubo

    (University of California)

  • Esther Wing Kwan Mak

    (University of California)

  • Peter K. Weber

    (Lawrence Livermore National Laboratory)

  • Xavier Mayali

    (Lawrence Livermore National Laboratory)

  • Jonathan P. Zehr

    (University of California)

Abstract

Biological nitrogen fixation is a major important source of nitrogen for low-nutrient surface oceanic waters. Nitrogen-fixing (diazotrophic) cyanobacteria are believed to be the primary contributors to this process, but the contribution of non-cyanobacterial diazotrophic organisms in oxygenated surface water, while hypothesized to be important, has yet to be demonstrated. In this study, we used simultaneous 15N-dinitrogen and 13C-bicarbonate incubations combined with nanoscale secondary ion mass spectrometry analysis to screen tens of thousands of mostly particle-associated, cell-like regions of interest collected from the North Pacific Subtropical Gyre. These dual isotope incubations allow us to distinguish between non-cyanobacterial and cyanobacterial nitrogen-fixing microorganisms and to measure putative cell-specific nitrogen fixation rates. With this approach, we detect nitrogen fixation by putative non-cyanobacterial diazotrophs in the oxygenated surface ocean, which are associated with organic-rich particles (

Suggested Citation

  • Katie J. Harding & Kendra A. Turk-Kubo & Esther Wing Kwan Mak & Peter K. Weber & Xavier Mayali & Jonathan P. Zehr, 2022. "Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34585-y
    DOI: 10.1038/s41467-022-34585-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-34585-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-34585-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Christopher R. Schvarcz & Samuel T. Wilson & Mathieu Caffin & Rosalina Stancheva & Qian Li & Kendra A. Turk-Kubo & Angelicque E. White & David M. Karl & Jonathan P. Zehr & Grieg F. Steward, 2022. "Overlooked and widespread pennate diatom-diazotroph symbioses in the sea," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Juan José Pierella Karlusich & Eric Pelletier & Fabien Lombard & Madeline Carsique & Etienne Dvorak & Sébastien Colin & Marc Picheral & Francisco M. Cornejo-Castillo & Silvia G. Acinas & Rainer Pepper, 2021. "Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Christopher R. Schvarcz & Samuel T. Wilson & Mathieu Caffin & Rosalina Stancheva & Qian Li & Kendra A. Turk-Kubo & Angelicque E. White & David M. Karl & Jonathan P. Zehr & Grieg F. Steward, 2022. "Overlooked and widespread pennate diatom-diazotroph symbioses in the sea," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Benedikt Ehrenfels & Kathrin B. L. Baumann & Robert Niederdorfer & Athanasio S. Mbonde & Ismael A. Kimirei & Thomas Kuhn & Paul M. Magyar & Daniel Odermatt & Carsten J. Schubert & Helmut Bürgmann & Mo, 2023. "Hydrodynamic regimes modulate nitrogen fixation and the mode of diazotrophy in Lake Tanganyika," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Xiyang Dong & Chuwen Zhang & Yongyi Peng & Hong-Xi Zhang & Ling-Dong Shi & Guangshan Wei & Casey R. J. Hubert & Yong Wang & Chris Greening, 2022. "Phylogenetically and catabolically diverse diazotrophs reside in deep-sea cold seep sediments," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34585-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.