IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-31796-1.html
   My bibliography  Save this article

Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

Author

Listed:
  • Kenneth Danh

    (Enable Biosciences Inc)

  • Donna Grace Karp

    (Enable Biosciences Inc)

  • Malvika Singhal

    (Enable Biosciences Inc)

  • Akshaya Tankasala

    (Enable Biosciences Inc)

  • David Gebhart

    (Enable Biosciences Inc)

  • Felipe Jesus Cortez

    (Enable Biosciences Inc)

  • Devangkumar Tandel

    (Enable Biosciences Inc)

  • Peter V. Robinson

    (Enable Biosciences Inc)

  • David Seftel

    (Enable Biosciences Inc)

  • Mars Stone

    (Vitalant Research Institute)

  • Graham Simmons

    (Vitalant Research Institute)

  • Anil Bagri

    (Cerus Corporation)

  • Martin A. Schreiber

    (Oregon Health & Science University)

  • Andreas Buser

    (University Hospital Basel, University of Basel)

  • Andreas Holbro

    (University Hospital Basel, University of Basel)

  • Manuel Battegay

    (University Hospital Basel, University of Basel)

  • Mary Kate Morris

    (California Department of Public Health)

  • Carl Hanson

    (California Department of Public Health)

  • John R. Mills

    (Mayo Clinic)

  • Dane Granger

    (Mayo Clinic)

  • Elitza S. Theel

    (Mayo Clinic)

  • James R. Stubbs

    (Mayo Clinic)

  • Laurence M. Corash

    (Cerus Corporation)

  • Cheng-ting Tsai

    (Enable Biosciences Inc)

Abstract

An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91–97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.

Suggested Citation

  • Kenneth Danh & Donna Grace Karp & Malvika Singhal & Akshaya Tankasala & David Gebhart & Felipe Jesus Cortez & Devangkumar Tandel & Peter V. Robinson & David Seftel & Mars Stone & Graham Simmons & Anil, 2022. "Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31796-1
    DOI: 10.1038/s41467-022-31796-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-31796-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-31796-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Fabian Schmidt & Yiska Weisblum & Magdalena Rutkowska & Daniel Poston & Justin DaSilva & Fengwen Zhang & Eva Bednarski & Alice Cho & Dennis J. Schaefer-Babajew & Christian Gaebler & Marina Caskey & Mi, 2021. "High genetic barrier to SARS-CoV-2 polyclonal neutralizing antibody escape," Nature, Nature, vol. 600(7889), pages 512-516, December.
    2. Stefania Dispinseri & Massimiliano Secchi & Maria Franca Pirillo & Monica Tolazzi & Martina Borghi & Cristina Brigatti & Maria Laura Angelis & Marco Baratella & Elena Bazzigaluppi & Giulietta Venturi , 2021. "Neutralizing antibody responses to SARS-CoV-2 in symptomatic COVID-19 is persistent and critical for survival," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Dennis Lapuente & Jana Fuchs & Jonas Willar & Ana Vieira Antão & Valentina Eberlein & Nadja Uhlig & Leila Issmail & Anna Schmidt & Friederike Oltmanns & Antonia Sophia Peter & Sandra Mueller-Schmucker, 2021. "Protective mucosal immunity against SARS-CoV-2 after heterologous systemic prime-mucosal boost immunization," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Dapeng Li & David R. Martinez & Alexandra Schäfer & Haiyan Chen & Maggie Barr & Laura L. Sutherland & Esther Lee & Robert Parks & Dieter Mielke & Whitney Edwards & Amanda Newman & Kevin W. Bock & Mahn, 2022. "Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Grace Kenny & Sophie O’Reilly & Neil Wrigley Kelly & Riya Negi & Colette Gaillard & Dana Alalwan & Gurvin Saini & Tamara Alrawahneh & Nathan Francois & Matthew Angeliadis & Alejandro Abner Garcia Leon, 2023. "Distinct receptor binding domain IgG thresholds predict protective host immunity across SARS-CoV-2 variants and time," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. David J. Sullivan & Massimo Franchini & Michael J. Joyner & Arturo Casadevall & Daniele Focosi, 2022. "Analysis of anti-SARS-CoV-2 Omicron-neutralizing antibody titers in different vaccinated and unvaccinated convalescent plasma sources," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    5. Sun Jin Kim & Zhong Yao & Morgan C. Marsh & Debra M. Eckert & Michael S. Kay & Anna Lyakisheva & Maria Pasic & Aiyush Bansal & Chaim Birnboim & Prabhat Jha & Yannick Galipeau & Marc-André Langlois & J, 2022. "Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    6. Simone Lanini & Stefano Milleri & Emanuele Andreano & Sarah Nosari & Ida Paciello & Giulia Piccini & Alessandra Gentili & Adhuna Phogat & Inesa Hyseni & Margherita Leonardi & Alessandro Torelli & Eman, 2022. "Safety and serum distribution of anti-SARS-CoV-2 monoclonal antibody MAD0004J08 after intramuscular injection," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    7. Milja Belik & Pinja Jalkanen & Rickard Lundberg & Arttu Reinholm & Larissa Laine & Elina Väisänen & Marika Skön & Paula A. Tähtinen & Lauri Ivaska & Sari H. Pakkanen & Hanni K. Häkkinen & Eeva Ortamo , 2022. "Comparative analysis of COVID-19 vaccine responses and third booster dose-induced neutralizing antibodies against Delta and Omicron variants," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    8. Leander Witte & Viren A. Baharani & Fabian Schmidt & Zijun Wang & Alice Cho & Raphael Raspe & Camila Guzman-Cardozo & Frauke Muecksch & Marie Canis & Debby J. Park & Christian Gaebler & Marina Caskey , 2023. "Epistasis lowers the genetic barrier to SARS-CoV-2 neutralizing antibody escape," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    9. Noemia S. Lima & Maryam Musayev & Timothy S. Johnston & Danielle A. Wagner & Amy R. Henry & Lingshu Wang & Eun Sung Yang & Yi Zhang & Kevina Birungi & Walker P. Black & Sijy O’Dell & Stephen D. Schmid, 2022. "Primary exposure to SARS-CoV-2 variants elicits convergent epitope specificities, immunoglobulin V gene usage and public B cell clones," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31796-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.