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Proteolysis of adaptor protein Mmr1 during budding is necessary for mitochondrial homeostasis in Saccharomyces cerevisiae

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  • Keisuke Obara

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Taku Yoshikawa

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Ryu Yamaguchi

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Keiko Kuwata

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Kunio Nakatsukasa

    (Nagoya City University)

  • Kohei Nishimura

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Takumi Kamura

    (Nagoya University, Furo-cho, Chikusa-ku)

Abstract

In yeast, mitochondria are passed on to daughter cells via the actin cable, motor protein Myo2, and adaptor protein Mmr1. They are released from the actin-myosin machinery after reaching the daughter cells. We report that Mmr1 is rapidly degraded by the ubiquitin-proteasome system in Saccharomyces cerevisiae. Redundant ubiquitin ligases Dma1 and Dma2 are responsible for Mmr1 ubiquitination. Dma1/2-mediated Mmr1 ubiquitination requires phosphorylation, most likely at S414 residue by Ste20 and Cla4. These kinases are mostly localized to the growing bud and nearly absent from mother cells, ensuring phosphorylation and ubiquitination of Mmr1 after the mitochondria enter the growing bud. In dma1Δ dma2Δ cells, transported mitochondria are first stacked at the bud-tip and then pulled back to the bud-neck. Stacked mitochondria in dma1Δ dma2Δ cells exhibit abnormal morphology, elevated respiratory activity, and increased level of reactive oxygen species, along with hypersensitivity to oxidative stresses. Collectively, spatiotemporally-regulated Mmr1 turnover guarantees mitochondrial homeostasis.

Suggested Citation

  • Keisuke Obara & Taku Yoshikawa & Ryu Yamaguchi & Keiko Kuwata & Kunio Nakatsukasa & Kohei Nishimura & Takumi Kamura, 2022. "Proteolysis of adaptor protein Mmr1 during budding is necessary for mitochondrial homeostasis in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29704-8
    DOI: 10.1038/s41467-022-29704-8
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    2. Sina Ghaemmaghami & Won-Ki Huh & Kiowa Bower & Russell W. Howson & Archana Belle & Noah Dephoure & Erin K. O'Shea & Jonathan S. Weissman, 2003. "Global analysis of protein expression in yeast," Nature, Nature, vol. 425(6959), pages 737-741, October.
    3. Wolfgang M. Pernice & Jason D. Vevea & Liza A. Pon, 2016. "A role for Mfb1p in region-specific anchorage of high-functioning mitochondria and lifespan in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 7(1), pages 1-12, April.
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