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Isolation of infectious Lloviu virus from Schreiber’s bats in Hungary

Author

Listed:
  • Gábor Kemenesi

    (University of Pécs
    University of Pécs)

  • Gábor E. Tóth

    (University of Pécs
    University of Pécs)

  • Martin Mayora-Neto

    (Universities of Kent & Greenwich)

  • Simon Scott

    (Universities of Kent & Greenwich)

  • Nigel Temperton

    (Universities of Kent & Greenwich)

  • Edward Wright

    (University of Sussex, Falmer)

  • Elke Mühlberger

    (Boston University School of Medicine)

  • Adam J. Hume

    (Boston University School of Medicine)

  • Ellen L. Suder

    (Boston University School of Medicine)

  • Brigitta Zana

    (University of Pécs)

  • Sándor A. Boldogh

    (Aggtelek National Park Directorate)

  • Tamás Görföl

    (University of Pécs)

  • Péter Estók

    (Eszterházy Károly University)

  • Tamara Szentiványi

    (Institute of Ecology and Botany, ÖK Centre for Ecological Research)

  • Zsófia Lanszki

    (University of Pécs
    University of Pécs)

  • Balázs A. Somogyi

    (University of Pécs)

  • Ágnes Nagy

    (Hungarian Defence Forces)

  • Csaba I. Pereszlényi

    (Hungarian Defence Forces)

  • Gábor Dudás

    (Hungarian Defence Forces)

  • Fanni Földes

    (University of Pécs)

  • Kornélia Kurucz

    (University of Pécs
    University of Pécs)

  • Mónika Madai

    (University of Pécs)

  • Safia Zeghbib

    (University of Pécs)

  • Piet Maes

    (Laboratory of Clinical and Epidemiological Virology)

  • Bert Vanmechelen

    (Laboratory of Clinical and Epidemiological Virology)

  • Ferenc Jakab

    (University of Pécs
    University of Pécs)

Abstract

Some filoviruses can be transmitted to humans by zoonotic spillover events from their natural host and filovirus outbreaks have occured with increasing frequency in the last years. The filovirus Lloviu virus (LLOV), was identified in 2002 in Schreiber’s bats (Miniopterus schreibersii) in Spain and was subsequently detected in bats in Hungary. Here we isolate infectious LLOV from the blood of a live sampled Schreiber’s bat in Hungary. The isolate is subsequently sequenced and cultured in the Miniopterus sp. kidney cell line SuBK12-08. It is furthermore able to infect monkey and human cells, suggesting that LLOV might have spillover potential. A multi-year surveillance of LLOV in bats in Hungary detects LLOV RNA in both deceased and live animals as well as in coupled ectoparasites from the families Nycteribiidae and Ixodidae. This correlates with LLOV seropositivity in sampled Schreiber’s bats. Our data support the role of bats, specifically Miniopterus schreibersii as hosts for LLOV in Europe. We suggest that bat-associated parasites might play a role in the natural ecology of filoviruses in temperate climate regions compared to filoviruses in the tropics.

Suggested Citation

  • Gábor Kemenesi & Gábor E. Tóth & Martin Mayora-Neto & Simon Scott & Nigel Temperton & Edward Wright & Elke Mühlberger & Adam J. Hume & Ellen L. Suder & Brigitta Zana & Sándor A. Boldogh & Tamás Görföl, 2022. "Isolation of infectious Lloviu virus from Schreiber’s bats in Hungary," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29298-1
    DOI: 10.1038/s41467-022-29298-1
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    References listed on IDEAS

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