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Emerging technologies and infection models in cellular microbiology

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  • Ana Teresa López-Jiménez

    (London School of Hygiene and Tropical Medicine)

  • Serge Mostowy

    (London School of Hygiene and Tropical Medicine)

Abstract

The field of cellular microbiology, rooted in the co-evolution of microbes and their hosts, studies intracellular pathogens and their manipulation of host cell machinery. In this review, we highlight emerging technologies and infection models that recently promoted opportunities in cellular microbiology. We overview the explosion of microscopy techniques and how they reveal unprecedented detail at the host-pathogen interface. We discuss the incorporation of robotics and artificial intelligence to image-based screening modalities, biochemical mapping approaches, as well as dual RNA-sequencing techniques. Finally, we describe chips, organoids and animal models used to dissect biophysical and in vivo aspects of the infection process. As our knowledge of the infected cell improves, cellular microbiology holds great promise for development of anti-infective strategies with translational applications in human health.

Suggested Citation

  • Ana Teresa López-Jiménez & Serge Mostowy, 2021. "Emerging technologies and infection models in cellular microbiology," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26641-w
    DOI: 10.1038/s41467-021-26641-w
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    as
    1. Simone Becattini & Eric R. Littmann & Ruth Seok & Luigi Amoretti & Emily Fontana & Roberta Wright & Mergim Gjonbalaj & Ingrid M. Leiner & George Plitas & Tobias M. Hohl & Eric G. Pamer, 2020. "Enhancing mucosal immunity by transient microbiota depletion," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    2. Bozena Mika-Gospodorz & Suparat Giengkam & Alexander J. Westermann & Jantana Wongsantichon & Willow Kion-Crosby & Suthida Chuenklin & Loo Chien Wang & Piyanate Sunyakumthorn & Radoslaw M. Sobota & Sel, 2020. "Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    3. Isabella Santi & Neeraj Dhar & Djenet Bousbaine & Yuichi Wakamoto & John D. McKinney, 2013. "Erratum: Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria," Nature Communications, Nature, vol. 4(1), pages 1-1, December.
    4. Yu Imai & Kirsten J. Meyer & Akira Iinishi & Quentin Favre-Godal & Robert Green & Sylvie Manuse & Mariaelena Caboni & Miho Mori & Samantha Niles & Meghan Ghiglieri & Chandrashekhar Honrao & Xiaoyu Ma , 2019. "A new antibiotic selectively kills Gram-negative pathogens," Nature, Nature, vol. 576(7787), pages 459-464, December.
    5. Jacqueline M. Kimmey & Jeremy P. Huynh & Leslie A. Weiss & Sunmin Park & Amal Kambal & Jayanta Debnath & Herbert W. Virgin & Christina L. Stallings, 2015. "Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection," Nature, Nature, vol. 528(7583), pages 565-569, December.
    6. Lauren K. Yum & Mariana X. Byndloss & Sanford H. Feldman & Hervé Agaisse, 2019. "Critical role of bacterial dissemination in an infant rabbit model of bacillary dysentery," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    7. Elsje G. Otten & Emma Werner & Ana Crespillo-Casado & Keith B. Boyle & Vimisha Dharamdasani & Claudio Pathe & Balaji Santhanam & Felix Randow, 2021. "Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection," Nature, Nature, vol. 594(7861), pages 111-116, June.
    8. M. Basler & M. Pilhofer & G. P. Henderson & G. J. Jensen & J. J. Mekalanos, 2012. "Type VI secretion requires a dynamic contractile phage tail-like structure," Nature, Nature, vol. 483(7388), pages 182-186, March.
    9. Isabella Santi & Neeraj Dhar & Djenet Bousbaine & Yuichi Wakamoto & John D. McKinney, 2013. "Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria," Nature Communications, Nature, vol. 4(1), pages 1-11, December.
    10. Olivier Disson & Solène Grayo & Eugénie Huillet & Georgios Nikitas & Francina Langa-Vives & Olivier Dussurget & Marie Ragon & Alban Le Monnier & Charles Babinet & Pascale Cossart & Marc Lecuit, 2008. "Conjugated action of two species-specific invasion proteins for fetoplacental listeriosis," Nature, Nature, vol. 455(7216), pages 1114-1118, October.
    11. Damián Lobato-Márquez & Jingwei Xu & Gizem Özbaykal Güler & Adaobi Ojiakor & Martin Pilhofer & Serge Mostowy, 2021. "Mechanistic insight into bacterial entrapment by septin cage reconstitution," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    12. Matthew D. Welch & Akihiro Iwamatsu & Timothy J. Mitchison, 1997. "Actin polymerization is induced by Arp 2/3 protein complex at the surface of Listeria monocytogenes," Nature, Nature, vol. 385(6613), pages 265-269, January.
    13. Andrew S. Moore & Stephen M. Coscia & Cory L. Simpson & Fabian E. Ortega & Eric C. Wait & John M. Heddleston & Jeffrey J. Nirschl & Christopher J. Obara & Pedro Guedes-Dias & C. Alexander Boecker & Te, 2021. "Actin cables and comet tails organize mitochondrial networks in mitosis," Nature, Nature, vol. 591(7851), pages 659-664, March.
    14. Fabian U. Zwettler & Sebastian Reinhard & Davide Gambarotto & Toby D. M. Bell & Virginie Hamel & Paul Guichard & Markus Sauer, 2020. "Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
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    1. Ana T. López-Jiménez & Gizem Özbaykal Güler & Serge Mostowy, 2024. "The great escape: a Shigella effector unlocks the septin cage," Nature Communications, Nature, vol. 15(1), pages 1-3, December.

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