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Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism

Author

Listed:
  • Julie Massart

    (Karolinska Institutet)

  • Rasmus J. O. Sjögren

    (Karolinska Institutet)

  • Brendan Egan

    (Karolinska Institutet
    Dublin City University)

  • Christian Garde

    (University of Copenhagen)

  • Magnus Lindgren

    (Karolinska Institutet)

  • Weifeng Gu

    (University of Massachusetts Medical School
    Department of Cell Biology and Neuroscience, University of California at Riverside)

  • Duarte M. S. Ferreira

    (Karolinska Institutet)

  • Mutsumi Katayama

    (Karolinska Institutet)

  • Jorge L. Ruas

    (Karolinska Institutet)

  • Romain Barrès

    (University of Copenhagen)

  • Donal J. O’Gorman

    (Dublin City University)

  • Juleen R. Zierath

    (Karolinska Institutet
    Karolinska Institutet
    University of Copenhagen)

  • Anna Krook

    (Karolinska Institutet)

Abstract

Skeletal muscle is a highly adaptable tissue and remodels in response to exercise training. Using short RNA sequencing, we determine the miRNA profile of skeletal muscle from healthy male volunteers before and after a 14-day aerobic exercise training regime. Among the exercise training-responsive miRNAs identified, miR-19b-3p was selected for further validation. Overexpression of miR-19b-3p in human skeletal muscle cells increases insulin signaling, glucose uptake, and maximal oxygen consumption, recapitulating the adaptive response to aerobic exercise training. Overexpression of miR-19b-3p in mouse flexor digitorum brevis muscle enhances contraction-induced glucose uptake, indicating that miR-19b-3p exerts control on exercise training-induced adaptations in skeletal muscle. Potential targets of miR-19b-3p that are reduced after aerobic exercise training include KIF13A, MAPK6, RNF11, and VPS37A. Amongst these, RNF11 silencing potentiates glucose uptake in human skeletal muscle cells. Collectively, we identify miR-19b-3p as an aerobic exercise training-induced miRNA that regulates skeletal muscle glucose metabolism.

Suggested Citation

  • Julie Massart & Rasmus J. O. Sjögren & Brendan Egan & Christian Garde & Magnus Lindgren & Weifeng Gu & Duarte M. S. Ferreira & Mutsumi Katayama & Jorge L. Ruas & Romain Barrès & Donal J. O’Gorman & Ju, 2021. "Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26095-0
    DOI: 10.1038/s41467-021-26095-0
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    References listed on IDEAS

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    1. Victor Ambros, 2004. "The functions of animal microRNAs," Nature, Nature, vol. 431(7006), pages 350-355, September.
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