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A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge

Author

Listed:
  • Yfat Yahalom-Ronen

    (Israel Institute for Biological Research)

  • Hadas Tamir

    (Israel Institute for Biological Research)

  • Sharon Melamed

    (Israel Institute for Biological Research)

  • Boaz Politi

    (Israel Institute for Biological Research)

  • Ohad Shifman

    (Israel Institute for Biological Research)

  • Hagit Achdout

    (Israel Institute for Biological Research)

  • Einat B. Vitner

    (Israel Institute for Biological Research)

  • Ofir Israeli

    (Israel Institute for Biological Research)

  • Elad Milrot

    (Israel Institute for Biological Research)

  • Dana Stein

    (Israel Institute for Biological Research)

  • Inbar Cohen-Gihon

    (Israel Institute for Biological Research)

  • Shlomi Lazar

    (Israel Institute for Biological Research)

  • Hila Gutman

    (Israel Institute for Biological Research)

  • Itai Glinert

    (Israel Institute for Biological Research)

  • Lilach Cherry

    (Israel Institute for Biological Research)

  • Yaron Vagima

    (Israel Institute for Biological Research)

  • Shirley Lazar

    (Israel Institute for Biological Research)

  • Shay Weiss

    (Israel Institute for Biological Research)

  • Amir Ben-Shmuel

    (Israel Institute for Biological Research)

  • Roy Avraham

    (Israel Institute for Biological Research)

  • Reut Puni

    (Israel Institute for Biological Research)

  • Edith Lupu

    (Israel Institute for Biological Research)

  • Elad Bar-David

    (Israel Institute for Biological Research)

  • Assa Sittner

    (Israel Institute for Biological Research)

  • Noam Erez

    (Israel Institute for Biological Research)

  • Ran Zichel

    (Israel Institute for Biological Research)

  • Emanuelle Mamroud

    (Israel Institute for Biological Research)

  • Ohad Mazor

    (Israel Institute for Biological Research)

  • Haim Levy

    (Israel Institute for Biological Research)

  • Orly Laskar

    (Israel Institute for Biological Research)

  • Shmuel Yitzhaki

    (Israel Institute for Biological Research)

  • Shmuel C. Shapira

    (Israel Institute for Biological Research)

  • Anat Zvi

    (Israel Institute for Biological Research)

  • Adi Beth-Din

    (Israel Institute for Biological Research)

  • Nir Paran

    (Israel Institute for Biological Research)

  • Tomer Israely

    (Israel Institute for Biological Research)

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-∆G-spike vaccine, in which the glycoprotein of VSV is replaced by the spike protein of SARS-CoV-2. In-vitro characterization of this vaccine indicates the expression and presentation of the spike protein on the viral membrane with antigenic similarity to SARS-CoV-2. A golden Syrian hamster in-vivo model for COVID-19 is implemented. We show that a single-dose vaccination results in a rapid and potent induction of SARS-CoV-2 neutralizing antibodies. Importantly, vaccination protects hamsters against SARS-CoV-2 challenge, as demonstrated by the abrogation of body weight loss, and alleviation of the extensive tissue damage and viral loads in lungs and nasal turbinates. Taken together, we suggest the recombinant VSV-∆G-spike as a safe, efficacious and protective vaccine against SARS-CoV-2.

Suggested Citation

  • Yfat Yahalom-Ronen & Hadas Tamir & Sharon Melamed & Boaz Politi & Ohad Shifman & Hagit Achdout & Einat B. Vitner & Ofir Israeli & Elad Milrot & Dana Stein & Inbar Cohen-Gihon & Shlomi Lazar & Hila Gut, 2020. "A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20228-7
    DOI: 10.1038/s41467-020-20228-7
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    Cited by:

    1. Ma’ayan Israeli & Yaara Finkel & Yfat Yahalom-Ronen & Nir Paran & Theodor Chitlaru & Ofir Israeli & Inbar Cohen-Gihon & Moshe Aftalion & Reut Falach & Shahar Rotem & Uri Elia & Ital Nemet & Limor Klik, 2022. "Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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