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Identification of region-specific astrocyte subtypes at single cell resolution

Author

Listed:
  • Mykhailo Y. Batiuk

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • Araks Martirosyan

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • Jérôme Wahis

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • Filip de Vin

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • Catherine Marneffe

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • Carola Kusserow

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • Jordan Koeppen

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience)

  • João Filipe Viana

    (University of Minho
    ICVS/3Bs - PT Government Associate Laboratory)

  • João Filipe Oliveira

    (University of Minho
    ICVS/3Bs - PT Government Associate Laboratory
    IPCA Campus)

  • Thierry Voet

    (Wellcome Trust Sanger Institute
    KU Leuven Department of Human Genetics)

  • Chris P. Ponting

    (Wellcome Trust Sanger Institute
    University of Oxford
    University of Edinburgh)

  • T. Grant Belgard

    (University of Oxford
    The Bioinformatics CRO)

  • Matthew G. Holt

    (VIB-KU Leuven Center for Brain and Disease Research
    KU Leuven Department of Neuroscience
    Leuven Brain Institute, KU Leuven)

Abstract

Astrocytes, a major cell type found throughout the central nervous system, have general roles in the modulation of synapse formation and synaptic transmission, blood–brain barrier formation, and regulation of blood flow, as well as metabolic support of other brain resident cells. Crucially, emerging evidence shows specific adaptations and astrocyte-encoded functions in regions, such as the spinal cord and cerebellum. To investigate the true extent of astrocyte molecular diversity across forebrain regions, we used single-cell RNA sequencing. Our analysis identifies five transcriptomically distinct astrocyte subtypes in adult mouse cortex and hippocampus. Validation of our data in situ reveals distinct spatial positioning of defined subtypes, reflecting the distribution of morphologically and physiologically distinct astrocyte populations. Our findings are evidence for specialized astrocyte subtypes between and within brain regions. The data are available through an online database (https://holt-sc.glialab.org/), providing a resource on which to base explorations of local astrocyte diversity and function in the brain.

Suggested Citation

  • Mykhailo Y. Batiuk & Araks Martirosyan & Jérôme Wahis & Filip de Vin & Catherine Marneffe & Carola Kusserow & Jordan Koeppen & João Filipe Viana & João Filipe Oliveira & Thierry Voet & Chris P. Pontin, 2020. "Identification of region-specific astrocyte subtypes at single cell resolution," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14198-8
    DOI: 10.1038/s41467-019-14198-8
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    Cited by:

    1. Irene Serra & Julio Esparza & Laura Delgado & Cristina Martín-Monteagudo & Margalida Puigròs & Petar Podlesniy & Ramón Trullás & Marta Navarrete, 2022. "Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Hidenori Tabata & Megumi Sasaki & Masakazu Agetsuma & Hitomi Sano & Yuki Hirota & Michio Miyajima & Kanehiro Hayashi & Takao Honda & Masashi Nishikawa & Yutaka Inaguma & Hidenori Ito & Hirohide Takeba, 2022. "Erratic and blood vessel-guided migration of astrocyte progenitors in the cerebral cortex," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    3. Enrica Boda & Martina Lorenzati & Roberta Parolisi & Brian Harding & Gianmarco Pallavicini & Luca Bonfanti & Amanda Moccia & Stephanie Bielas & Ferdinando Di Cunto & Annalisa Buffo, 2022. "Molecular and functional heterogeneity in dorsal and ventral oligodendrocyte progenitor cells of the mouse forebrain in response to DNA damage," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    4. Jingyang Qian & Jie Liao & Ziqi Liu & Ying Chi & Yin Fang & Yanrong Zheng & Xin Shao & Bingqi Liu & Yongjin Cui & Wenbo Guo & Yining Hu & Hudong Bao & Penghui Yang & Qian Chen & Mingxiao Li & Bing Zha, 2023. "Reconstruction of the cell pseudo-space from single-cell RNA sequencing data with scSpace," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    5. Junjun Jing & Jifan Feng & Yuan Yuan & Tingwei Guo & Jie Lei & Fei Pei & Thach-Vu Ho & Yang Chai, 2022. "Spatiotemporal single-cell regulatory atlas reveals neural crest lineage diversification and cellular function during tooth morphogenesis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    6. Ravneet Jaura & Ssu-Yu Yeh & Kaitlin N. Montanera & Alyssa Ialongo & Zobia Anwar & Yiming Lu & Kavindu Puwakdandawa & Ho Sung Rhee, 2022. "Extended intergenic DNA contributes to neuron-specific expression of neighboring genes in the mammalian nervous system," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    7. Juan F. Quintana & Praveena Chandrasegaran & Matthew C. Sinton & Emma M. Briggs & Thomas D. Otto & Rhiannon Heslop & Calum Bentley-Abbot & Colin Loney & Luis de Lecea & Neil A. Mabbott & Annette MacLe, 2022. "Single cell and spatial transcriptomic analyses reveal microglia-plasma cell crosstalk in the brain during Trypanosoma brucei infection," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    8. Marc Duque & Corinne A. Lee-Kubli & Yusuf Tufail & Uri Magaram & Janki Patel & Ahana Chakraborty & Jose Mendoza Lopez & Eric Edsinger & Aditya Vasan & Rani Shiao & Connor Weiss & James Friend & Sreeka, 2022. "Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    9. Samuel S. Kim & Buu Truong & Karthik Jagadeesh & Kushal K. Dey & Amber Z. Shen & Soumya Raychaudhuri & Manolis Kellis & Alkes L. Price, 2024. "Leveraging single-cell ATAC-seq and RNA-seq to identify disease-critical fetal and adult brain cell types," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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