IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v15y2023i21p15406-d1269743.html
   My bibliography  Save this article

Inoculation with Biofilm of Bacillus subtilis Promotes the Growth of Lactuca sativa

Author

Listed:
  • Gabriela Cristina Sarti

    (Inorganic and Analitic Chemistry Cathedra, Department of Natural Resources and Environment, Faculty of Agronomy, University of Buenos Aires, Av. San Martín 4453, Buenos Aires C1417DSE, Argentina
    AQUATERRA Group, Interdisciplinary Center for Chemistry and Biology, CICA, As Carballeiras, s/n Campus de Elviña, University of A Coruna, 15008 Coruna, Spain)

  • Mirta Esther Galelli

    (Agrofood Area, Department of Applied Biology and Food, Faculty of Agronomy, University of Buenos Aires, Av. San Martín 4453, Buenos Aires C1417DSE, Argentina)

  • Silvana Arreghini

    (Inorganic and Analitic Chemistry Cathedra, Department of Natural Resources and Environment, Faculty of Agronomy, University of Buenos Aires, Av. San Martín 4453, Buenos Aires C1417DSE, Argentina)

  • Josefina Ana Eva Cristóbal-Miguez

    (Inorganic and Analitic Chemistry Cathedra, Department of Natural Resources and Environment, Faculty of Agronomy, University of Buenos Aires, Av. San Martín 4453, Buenos Aires C1417DSE, Argentina)

  • José Alfredo Curá

    (Biochemistry Cathedra, Department of Applied Biology and Food, Faculty of Agronomy, University of Buenos Aires, Av. San Martín 4453, Buenos Aires C1417DSE, Argentina)

  • Antonio Paz-González

    (AQUATERRA Group, Interdisciplinary Center for Chemistry and Biology, CICA, As Carballeiras, s/n Campus de Elviña, University of A Coruna, 15008 Coruna, Spain)

Abstract

In Argentina, the excessive use of fertilizers is common in intensively cultivated zones around highly populated areas. Bioinoculants based on plant growth promoting rhizobacteria (PGPR) could be effective for crop production improvement without negative effects on the environment. The objective of this work was to evaluate an alternative inoculation method, namely the application of the biofilm produced by Bacillus subtilis as a growth promoter on seeds of three varieties of Lactuca sativa , and to compare it with the common planktonic approach. Biofilm was obtained under static culture conditions, while planktonic inoculum was produced at 150 rpm. The major biofilm effects were observed with Bacillus subtilis subsp. spizizenii , that showed antifungal activity against phytopathogens, synthesized plant growth regulators (IAA, cytokinin and ABA) and solubilized phosphates. The Grand Rapid variety inoculated with biofilm showed the best results, with 30% and 37% higher aerial and root biomass, respectively, compared to the planktonic form. Moreover, the biofilm positive effects were observed through successive plant development stages until harvest, when the bacterium was recovered from the interior of the roots. The biofilm of B. subtilis subsp. spizizenii behave as a superior growth-promoting inoculant compared to the traditional planktonic inoculation technique.

Suggested Citation

  • Gabriela Cristina Sarti & Mirta Esther Galelli & Silvana Arreghini & Josefina Ana Eva Cristóbal-Miguez & José Alfredo Curá & Antonio Paz-González, 2023. "Inoculation with Biofilm of Bacillus subtilis Promotes the Growth of Lactuca sativa," Sustainability, MDPI, vol. 15(21), pages 1-16, October.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:21:p:15406-:d:1269743
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/15/21/15406/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/15/21/15406/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:15:y:2023:i:21:p:15406-:d:1269743. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.