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Commensal Pseudomonas Species Isolated from Wastewater and Freshwater Milieus in the Eastern Cape Province, South Africa, as Reservoir of Antibiotic Resistant Determinants

Author

Listed:
  • Isoken H. Igbinosa

    (Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Uchechukwu U. Nwodo

    (Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Anibal Sosa

    (Former Director, International Program & Clinical Advisor, Alliance for the Prudent Use of Antibiotics (APUA), 75 Kneeland Street, Boston, MA 02111, USA)

  • Mvuyo Tom

    (Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Anthony I. Okoh

    (Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

Abstract

Pseudomonas species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR) and extremely drug resistant (XDR) bacteria Pseudomonas species represent a threat to public health. Prevalence, antibiogram and associated antibiotic resistant genes of Pseudomonas species isolated from freshwater and mixed liquor environments in the Eastern Cape Province of South Africa were assessed. Polymerase chain reaction (PCR) based technique was used to identify the isolates and screen for antibiotic resistant genes. The result shows occurrence of Pseudomonas spp. in freshwater and mixed liquor as follows: 71.42% and 37.5% ( P. putida ), 14.28% and 31.25% ( P. flourescens ), 7.14% and 6.25% ( P. aeruginosa ) and 7.14% and 25% for other Pseudomonas species respectively. Disk diffusion antibiogram of the Pseudomonas isolates from the two locations showed 100% resistance to penicillin, oxacillin, clindamycin, rifampicin and 100% susceptibility to ciprofloxacin and gentamicin with varied percentage resistances to cephalothin, nalidixic acid, tetracycline, and ampicillin. The bla TEM antibiotic resistant gene was detected in 12.5% of P. putida , 57.14% of P. fluorescens , 100% P. aeruginosa and 40% in other Pseudomonas species. Similarly, Integrons conserved segment were detected in 12.5% of P. putida , 57.14% of P. fluorescens , 100% of P. aeruginosa and 40% of other Pseudomonas species. The presence of bla TEM gene and integrons conserved segment in some of the isolates is worrisome and suggest Pseudomona s species as important reservoirs of multidrug resistance genes in the Eastern Cape Province environment.

Suggested Citation

  • Isoken H. Igbinosa & Uchechukwu U. Nwodo & Anibal Sosa & Mvuyo Tom & Anthony I. Okoh, 2012. "Commensal Pseudomonas Species Isolated from Wastewater and Freshwater Milieus in the Eastern Cape Province, South Africa, as Reservoir of Antibiotic Resistant Determinants," IJERPH, MDPI, vol. 9(7), pages 1-13, July.
    • Handle: RePEc:gam:jijerp:v:9:y:2012:i:7:p:2537-2549:d:18994
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    References listed on IDEAS

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    1. Moira A. Gilliver & Malcolm Bennett & Michael Begon & Sarah M. Hazel & C. Anthony Hart, 1999. "Antibiotic resistance found in wild rodents," Nature, Nature, vol. 401(6750), pages 233-234, September.
    2. Jonathan K. Lutz & Jiyoung Lee, 2011. "Prevalence and Antimicrobial-Resistance of Pseudomonas aeruginosa in Swimming Pools and Hot Tubs," IJERPH, MDPI, vol. 8(2), pages 1-11, February.
    3. Monica Österblad & Kai Norrdahl & Erkki Korpimäki & Pentti Huovinen, 2001. "How wild are wild mammals?," Nature, Nature, vol. 409(6816), pages 37-38, January.
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