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Molecular Cloning and Characterization of a Serotonin N -Acetyltransferase Gene, xoSNAT3 , from Xanthomonas oryzae pv. oryzae

Author

Listed:
  • Xian Chen

    (State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection, Ministry of Agriculture and Rural Affairs, Zhejiang Provincial Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
    Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety—State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China)

  • Yancun Zhao

    (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety—State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China)

  • Pedro Laborda

    (School of Life Sciences, Nantong University, Nantong 226001, China)

  • Yong Yang

    (State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection, Ministry of Agriculture and Rural Affairs, Zhejiang Provincial Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China)

  • Fengquan Liu

    (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety—State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China)

Abstract

Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae ( Xoo ), is one of the top ten bacterial plant diseases worldwide. Serotonin N -acetyltransferase (SNAT) is one of the key rate-limiting enzymes in melatonin (MT) biosynthesis. However, its function in pathogenic bacteria remains unclear. In this study, a Xoo SNAT protein (xoSNAT3) that showed 27.39% homology with sheep SNAT was identified from a collection of 24 members of GCN5-related N -acetyltransferase (GNAT) superfamily in Xoo . This xoSNAT3 could be induced by MT. In tobacco-based transient expression system, xoSNAT3 was found localized on mitochondria. In vitro studies indicated that xoSNAT3 showed the optima enzymatic activity at 50 °C. The recombinant enzyme showed K m and V max values of 709.98 μM and 2.21 nmol/min/mg protein, respectively. Mutant △ xoSNAT3 showed greater impaired MT biosynthesis than the wild-type strain. Additionally, △ xoSNAT3 showed 14.06% less virulence and 26.07% less biofilm formation. Collectively, our results indicated that xoSNAT3 services as a SNAT involved in MT biosynthesis and pathogenicity in Xoo .

Suggested Citation

  • Xian Chen & Yancun Zhao & Pedro Laborda & Yong Yang & Fengquan Liu, 2023. "Molecular Cloning and Characterization of a Serotonin N -Acetyltransferase Gene, xoSNAT3 , from Xanthomonas oryzae pv. oryzae," IJERPH, MDPI, vol. 20(3), pages 1-16, January.
    • Handle: RePEc:gam:jijerp:v:20:y:2023:i:3:p:1865-:d:1041240
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