IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v19y2022i5p2954-d763249.html
   My bibliography  Save this article

In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts

Author

Listed:
  • María Pilar Pecci-Lloret

    (Gerodontology and Special Care Dentistry Unit, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain)

  • Sergio López-García

    (Department of Stomatology, Faculty of Medicine and Dentistry, Universitat de Valencia, 46010 Valencia, Spain
    Cellular Therapy and Hematopoietic Transplant Group, Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain)

  • Francisco Javier Rodríguez-Lozano

    (Gerodontology and Special Care Dentistry Unit, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
    Cellular Therapy and Hematopoietic Transplant Group, Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain)

  • Pablo Álvarez-Novoa

    (Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, 15705 La Coruña, Spain)

  • David García-Bernal

    (Cellular Therapy and Hematopoietic Transplant Group, Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain
    Biochemistry, Molecular Biology and Immunology Department, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain)

Abstract

The European Academy of Paediatric Dentistry has recommended fluoride toothpastes from the eruption of the first teeth in children. Toothpastes stay in the mouth in contact with human gingival fibroblasts (hGFs) for a long time. Thus, the objective of this study was to compare the cytotoxicity of five different commonly used children’s toothpastes: Oral B Kids +3 (Procter & Gamble, Alicante, Spain), Fluor Kin Calcium (Kin, Madrid, Spain), PHB Junior (PHB, Barcelona, Spain), Colgate +3 (Colgate Palmolive, Madrid, Spain) and Vitis Kids (Dentaid, Valencia, Spain) on hGFs. The children’s toothpastes were exposed to hGFs at different concentrations (1:1, 1:2, 1:4). Afterwards, several tests were performed: MTT assays, cell cycle analyses, cell cytoskeleton staining assays, apoptosis/necrosis assays, and ICP-MS and ion chromatography. Oral B displayed the lowest cytotoxicity and was the toothpaste with the highest fluoride ion release; meanwhile, the other toothpastes were cytotoxic (*** p < 0.0001); Fluor Kin being the one with the lowest fluoride ion release. Among all the toothpastes analyzed, Oral B exhibited the best results in vitro in terms of biocompatibility. Future evaluations, both in vitro and in vivo, are required to confirm the biocompatibility of sodium lauryl sarcosinate and sodium lauryl sulfate containing toothpastes.

Suggested Citation

  • María Pilar Pecci-Lloret & Sergio López-García & Francisco Javier Rodríguez-Lozano & Pablo Álvarez-Novoa & David García-Bernal, 2022. "In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts," IJERPH, MDPI, vol. 19(5), pages 1-11, March.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:5:p:2954-:d:763249
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/19/5/2954/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/19/5/2954/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:19:y:2022:i:5:p:2954-:d:763249. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.