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Inhibitory Effect of Monoterpenoid Glycosides Extracts from Peony Seed Meal on Streptococcus suis LuxS/AI-2 Quorum Sensing System and Biofilm

Author

Listed:
  • Jinpeng Li

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China
    Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China
    These authors contributed equally to this study.)

  • Yamin Shen

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China
    These authors contributed equally to this study.)

  • Jing Zuo

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Shuji Gao

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Haikun Wang

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Yuxin Wang

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Li Yi

    (Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China
    College of Life Science, Luoyang Normal University, Luoyang 471000, China)

  • Xiaogai Hou

    (College of Agriculture/College of Tree Peony, Henan University of Science and Technology, Luoyang 471000, China)

  • Yang Wang

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

Abstract

Streptococcus suis LuxS/AI-2 quorum sensing system regulates biofilm formation, resulting in increased pathogenicity and drug resistance, and diminished efficacy of antibiotic treatment. The remaining peony seed cake after oil extraction is rich in monoterpenoid glycosides, which can inhibit the formation of bacterial biofilm. In this study, we investigated the effect of seven major monocomponents (suffruticosol A, suffruticosol B, suffruticosol C, paeonifloin, albiflorin, trans-ε-viniferin, gnetin H) of peony seed meal on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of S. suis . The results showed that the MICs of the seven single components were all greater than 200 μg/mL, with no significant bacteriostatic and bactericidal advantages. Crystal violet staining and scanning electron microscope observation showed that the seven single components had a certain inhibitory effect on the biofilm formation ability of S. suis at sub-MIC concentration. Among them, the ability of paeoniflorin to inhibit biofilm was significantly higher than that of the other six single components. AI-2 signaling molecules were detected by bioreporter strain Vibrio harvey BB170 . The detection results of AI-2 signal molecules found that at 1/2 MIC concentration, paeoniflorin significantly inhibited the production of S. suis AI-2 signal, and the inhibitory effect was better than that of the other six single components. In addition, molecular docking analysis revealed that paeoniflorin had a significant binding activity with LuxS protein compared with the other six single components. The present study provides evidence that paeoniflorin plays a key role in the regulation of the inhibition of S. suis LuxS/AI-2 system and biofilm formation in peony seed meal.

Suggested Citation

  • Jinpeng Li & Yamin Shen & Jing Zuo & Shuji Gao & Haikun Wang & Yuxin Wang & Li Yi & Xiaogai Hou & Yang Wang, 2022. "Inhibitory Effect of Monoterpenoid Glycosides Extracts from Peony Seed Meal on Streptococcus suis LuxS/AI-2 Quorum Sensing System and Biofilm," IJERPH, MDPI, vol. 19(23), pages 1-11, November.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:23:p:16024-:d:989353
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